{"id":"65d6a556-5213-52e4-84c8-62258372d376","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-pparg-phosphorylation","predicate":"increases_phosphorylation","statement":"Adding t10,c12 CLA increased PPAR-gamma and ERK1/2 phosphorylation before PPAR-gamma protein declined.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b0ff2f87-92ec-533b-8eac-b7329f2f6e3a","mechanism_event_label":"Signaling changed before the regulator became less abundant.","subject":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"object":{"id":"7f456e18-fabc-532d-bbfe-cd3a8cf1b348","slug":"pparg","display_name":"Human PPAR gamma / PPARG","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"b0ff2f87-92ec-533b-8eac-b7329f2f6e3a","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-pparg-phosphorylation-event","event_type":"observed_relationship","label":"Signaling changed before the regulator became less abundant.","description":"Adding t10,c12 CLA increased PPAR-gamma and ERK1/2 phosphorylation before PPAR-gamma protein declined.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7f456e18-fabc-532d-bbfe-cd3a8cf1b348","slug":"pparg","display_name":"Human PPAR gamma / PPARG","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae89cec3-34fe-5d92-bdcf-b3c745f0f405","slug":"mapk1","display_name":"Human ERK2 / MAPK1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"1d07742f-1a59-5c14-b26b-3f1847be6999","slug":"mapk3","display_name":"Human ERK1 / MAPK3","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human adipocyte time-course experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"ERK causation of the PPAR-gamma phosphorylation was proposed rather than isolated here.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Signaling changed before the regulator became less abundant.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Trans-10, cis-12 conjugated linoleic acid antagonizes ligand-dependent PPARgamma activity in primary cultures of human adipocytes. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18287349/ · DOI 10.1093/jn/138.3.455","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5ee24b1f-5474-50c6-b486-87daa5fe2f68","evidence_kind":"source_excerpt","locator":"Lines 110-116","start_line":110,"end_line":116,"excerpt":"## cla-pparg-phosphorylation\nSignaling changed before the regulator became less abundant.\nAdding t10,c12 CLA increased PPAR-gamma and ERK1/2 phosphorylation before PPAR-gamma protein declined.\nModel: Human adipocyte time-course experiments.\nLimitations: ERK causation of the PPAR-gamma phosphorylation was proposed rather than isolated here.\nEvidence access: Primary abstract\nTrans-10, cis-12 conjugated linoleic acid antagonizes ligand-dependent PPARgamma activity in primary cultures of human adipocytes. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18287349/ · DOI 10.1093/jn/138.3.455","model_system":"Human adipocyte time-course experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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