{"id":"65bae628-d920-5194-98d1-1e55849e99f8","stable_key":"ea2f0904-ab2c-5d1e-a3d4-3f7ecdd70cd6:caf-p53","predicate":"reduces","statement":"Caffeine reduced radiation-induced p53 Ser15 phosphorylation; DNA-PK was resistant and Chk1 only marginally inhibited in the comparison.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"1e499af7-e0ba-5788-a521-6c2c554794e5","mechanism_event_label":"The response was selective across the tested kinase pathways.","subject":{"id":"ef028472-7c33-583d-b52e-40676004d370","slug":"caffeine","display_name":"Caffeine","entity_type_key":"small_molecule"},"object":{"id":"080981de-4f96-55db-9dee-71cb90b94013","slug":"p53-ser15-phosphorylation","display_name":"p53 Ser15 phosphorylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"1e499af7-e0ba-5788-a521-6c2c554794e5","stable_key":"ea2f0904-ab2c-5d1e-a3d4-3f7ecdd70cd6:caf-p53-event","event_type":"observed_relationship","label":"The response was selective across the tested kinase pathways.","description":"Caffeine reduced radiation-induced p53 Ser15 phosphorylation; DNA-PK was resistant and Chk1 only marginally inhibited in the comparison.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ef028472-7c33-583d-b52e-40676004d370","slug":"caffeine","display_name":"Caffeine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"080981de-4f96-55db-9dee-71cb90b94013","slug":"p53-ser15-phosphorylation","display_name":"p53 Ser15 phosphorylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"243ad948-2281-53f8-8e53-32247253668d","slug":"atm","display_name":"Human ATM kinase","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"fe8d147a-fd3c-5768-ae24-f6c4fbd2ae8d","slug":"atr","display_name":"Human ATR kinase","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Kinase and radiation-response assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not infer identical activity against all DNA-damage kinases.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Caffeine collection; salts, coffee, species and coexposure contexts retain their identities.","comparator":null,"unit":null,"notes":"","entity":{"slug":"caffeine","display_name":"Caffeine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The response was selective across the tested kinase pathways.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition of ATM and ATR kinase activities by the radiosensitizing agent, caffeine. · 1999 · https://pubmed.ncbi.nlm.nih.gov/10485486/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"84856e2f-fe08-52ae-9ea7-d82aedacdc3b","evidence_kind":"source_excerpt","locator":"Lines 436-442","start_line":436,"end_line":442,"excerpt":"## caf-p53\nThe response was selective across the tested kinase pathways.\nCaffeine reduced radiation-induced p53 Ser15 phosphorylation; DNA-PK was resistant and Chk1 only marginally inhibited in the comparison.\nModel: Kinase and radiation-response assays.\nLimitations: Do not infer identical activity against all DNA-damage kinases.\nEvidence access: Primary abstract\nInhibition of ATM and ATR kinase activities by the radiosensitizing agent, caffeine. · 1999 · https://pubmed.ncbi.nlm.nih.gov/10485486/","model_system":"Kinase and radiation-response assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access stated with the claim.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"40a654db-878c-5471-bc6c-88f4f52599c0","stable_key":"import-ea2f0904-ab2c-5d1e-a3d4-3f7ecdd70cd6","title":"Caffeine: receptors, metabolism, nutrient interactions, adaptation and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"780d73d4abd45a0284d2cc0c646dbe8432ae347362891a4b98a19a61341a7325","revision_id":"f36ffb0a-d6df-522f-b783-5e30214b9e26","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}