{"id":"6599223d-0e25-5a13-bee7-668d66ff72c0","stable_key":"research:gpx3-loss-increases-provoked-thrombosis","predicate":"loss-increases","statement":"Gpx3 deficiency increased platelet-dependent thrombosis after experimental vascular provocation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"positive","is_public":true,"mechanism_event_id":"89022c0f-c093-5a3a-afda-a29d5ddaea53","mechanism_event_label":"GPX3-deficient mice formed more thrombus after vascular injury.","subject":{"id":"32f7aa34-135f-5bfb-9189-81634ad4a824","slug":"gpx3","display_name":"GPX3","entity_type_key":"protein"},"object":{"id":"4b772fbf-8a5b-57cd-ad5d-f41634a4688c","slug":"arterial-thrombosis","display_name":"Arterial thrombosis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"89022c0f-c093-5a3a-afda-a29d5ddaea53","stable_key":"research:gpx3-loss-increases-provoked-thrombosis","event_type":"genetic-perturbation","label":"GPX3-deficient mice formed more thrombus after vascular injury.","description":"Gpx3 deficiency increased platelet-dependent thrombosis after experimental vascular provocation.","status":"active","compartment":{"slug":"vasculature","display_name":"Vasculature"},"participants":[{"entity":{"id":"32f7aa34-135f-5bfb-9189-81634ad4a824","slug":"gpx3","display_name":"GPX3","entity_type_key":"protein"},"role":"perturbed-protein","stoichiometry":null,"state_label":"deleted","sequence_order":0,"notes":""},{"entity":{"id":"4b772fbf-8a5b-57cd-ad5d-f41634a4688c","slug":"arterial-thrombosis","display_name":"Arterial thrombosis","entity_type_key":"cellular_process"},"role":"measured-process","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a83eed3d-6e9b-5730-b5aa-cc1448ee5337","slug":"platelet-activation","display_name":"Platelet activation","entity_type_key":"cellular_process"},"role":"participating-process","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"arterial injury model","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Gpx3 knockout","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not establish a human selenium cutoff.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"mouse","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c14cd652-d79b-506d-a3ad-a8e3030e3823","evidence_kind":"curated_literature_summary","locator":"lines 702-712","start_line":702,"end_line":712,"excerpt":"## gpx3-loss-increases-provoked-thrombosis\n\nGPX3-deficient mice formed more thrombus after vascular injury.\n\nGpx3 deficiency increased platelet-dependent thrombosis after experimental vascular provocation.\n\nOrganism: mouse\nCell type: arterial injury model\nExperimental model: Gpx3 knockout\nLimitations: Does not establish a human selenium cutoff.\nPrimary reference: [Glutathione Peroxidase-3 Deficiency Promotes Platelet-dependent Thrombosis in vivo](https://pmc.ncbi.nlm.nih.gov/articles/PMC3107543/)","model_system":"Gpx3 knockout","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"vascular-redox","plain_language":"GPX3-deficient mice formed more thrombus after vascular injury.","evidence_scope":"direct_experimental","papers":[{"key":"jin-2011-gpx3-platelets","title":"Glutathione Peroxidase-3 Deficiency Promotes Platelet-dependent Thrombosis in vivo","url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC3107543/","doi":"10.1161/CIRCULATIONAHA.110.000034","year":2011,"model":"Gpx3-knockout mice and platelet/vascular assays","summary":"Finds platelet hyperreactivity and increased thrombosis, with evidence implicating altered redox and nitric-oxide signaling."}]}}