{"id":"65774916-4939-5f4a-9b1e-2bc204766f96","stable_key":"c932089b-ebc9-5f39-b2e5-84af74f9f6d0:sucrose-sweet-receptor","predicate":"activates","statement":"Sucrose elicited a calcium response in cells coexpressing human TAS1R2 and TAS1R3, but not either subunit alone.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"9fac36da-7d67-588e-b895-883bd2736611","mechanism_event_label":"Sucrose elicited a calcium response in cells coexpressing human TAS1R2 and TAS1R3, but not either subunit alone.","subject":{"id":"82558bd0-1a84-574a-a04c-10609c9c5dba","slug":"sucrose","display_name":"Sucrose","entity_type_key":"small_molecule"},"object":{"id":"2eca6132-b441-5473-98bb-6759c0b2a5fc","slug":"human-tas1r2-tas1r3","display_name":"Human sweet taste receptor TAS1R2/TAS1R3","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"9fac36da-7d67-588e-b895-883bd2736611","stable_key":"c932089b-ebc9-5f39-b2e5-84af74f9f6d0:sucrose-sweet-receptor-event","event_type":"observed_relationship","label":"Sucrose elicited a calcium response in cells coexpressing human TAS1R2 and TAS1R3, but not either subunit alone.","description":"Sucrose elicited a calcium response in cells coexpressing human TAS1R2 and TAS1R3, but not either subunit alone.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"82558bd0-1a84-574a-a04c-10609c9c5dba","slug":"sucrose","display_name":"Sucrose","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2eca6132-b441-5473-98bb-6759c0b2a5fc","slug":"human-tas1r2-tas1r3","display_name":"Human sweet taste receptor TAS1R2/TAS1R3","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"09713aab-d5e3-589c-83c2-95d3989efd62","slug":"tas1r2","display_name":"Human taste receptor TAS1R2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7ab6f209-2089-5008-b64e-64f8db8c7771","slug":"tas1r3","display_name":"Human taste receptor TAS1R3","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"300 mM sucrose with or without 1.25 mM lactisole","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute calcium response","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Selected primary full-text sections inspected via indexed PMC page; local XML has abstract only.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human TAS1R2/TAS1R3 coexpressed with engineered G-alpha15 in HEK-derived cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure_scope","value_text":"Human receptor in an expression system","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Engineered coupling and high assay concentration do not measure human dietary absorption. Rat lactisole sensitivity differs. Selected full-text sections inspected; archived XML is abstract only.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sucrose chapter; direct sucrose observations are distinguished from shared component metabolism.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sucrose","display_name":"Sucrose","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human TAS1R2/TAS1R3 coexpressed with engineered G-alpha15 in HEK-derived cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Sucrose elicited a calcium response in cells coexpressing human TAS1R2 and TAS1R3, but not either subunit alone.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Human receptors for sweet and umami taste. (2002). https://pubmed.ncbi.nlm.nih.gov/11917125/ DOI: 10.1073/pnas.072090199","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro receptor stimulation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Recombinant sweet-receptor calcium-response assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cd9b0643-1fbc-5890-a5b8-cc9f564bd4e2","evidence_kind":"source_excerpt","locator":"Lines 55-65","start_line":55,"end_line":65,"excerpt":"## sucrose-sweet-receptor\nSucrose elicited a calcium response in cells coexpressing human TAS1R2 and TAS1R3, but not either subunit alone.\nModel/species: Human TAS1R2/TAS1R3 coexpressed with engineered G-alpha15 in HEK-derived cells\nTissue: Recombinant sweet-receptor calcium-response assay\nExposure: 300 mM sucrose with or without 1.25 mM lactisole\nRoute: In vitro receptor stimulation\nDuration: Acute calcium response\nExposure scope: Human receptor in an expression system\nLimits: Engineered coupling and high assay concentration do not measure human dietary absorption. Rat lactisole sensitivity differs. Selected full-text sections inspected; archived XML is abstract only.\nReference: Human receptors for sweet and umami taste. (2002). https://pubmed.ncbi.nlm.nih.gov/11917125/ DOI: 10.1073/pnas.072090199\nAccess: Selected primary full-text sections inspected via indexed PMC page; local XML has abstract only.","model_system":"Human TAS1R2/TAS1R3 coexpressed with engineered G-alpha15 in HEK-derived cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; provenance retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"8e24ddf4-84ee-57b4-9fd8-093547432ece","stable_key":"import-c932089b-ebc9-5f39-b2e5-84af74f9f6d0","title":"Sucrose: mechanism of action and metabolic impact (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific sucrose curation with shared canonical claims retained by identity. Primary-study citations, negative findings, exposure details and limitations preserved. Not publisher full text.","file_path":"","sha256":"5e68ccbade7a80fc03778495756f0eeeeba5038cd10d5bef8e33cb1e93e4b044","revision_id":"6edb8ffc-11c5-5cd9-852c-85adb71165a8","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}