{"id":"65697297-d895-5042-b251-6239108bdd88","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-gout-sirt3","predicate":"increases_in_recorded_comparison","statement":"Apigenin restored SIRT3 protein abundance and decreased acetylated SOD2 in MSU-challenged mouse macrophages.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"992dfc25-76f4-5dc4-be09-fa9adb6c8201","mechanism_event_label":"Apigenin restored SIRT3 protein abundance and decreased acetylated SOD2 in MSU-challenged mouse macrophages.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"b1f091bb-03fe-5067-8bd0-785e79a9c5ff","slug":"mouse-sirt3","display_name":"Mouse mitochondrial sirtuin 3 / Sirt3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"992dfc25-76f4-5dc4-be09-fa9adb6c8201","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-gout-sirt3-event","event_type":"experimental_observation","label":"Apigenin restored SIRT3 protein abundance and decreased acetylated SOD2 in MSU-challenged mouse macrophages.","description":"### api-gout-sirt3\n\nApigenin restored SIRT3 protein abundance and decreased acetylated SOD2 in MSU-challenged mouse macrophages.\nRestored SIRT3 protein is not a direct binding or activity assay.\nModel and exposure: Mouse BMDMs; 25 micromolar apigenin.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: The NADase CD38 is a central regulator in gouty inflammation and a novel druggable therapeutic target. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38493256/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11058052/ | https://doi.org/10.1007/s00011-024-01863-y | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Apigenin plus MSU","sequence_order":0,"notes":""},{"entity":{"id":"b1f091bb-03fe-5067-8bd0-785e79a9c5ff","slug":"mouse-sirt3","display_name":"Mouse mitochondrial sirtuin 3 / Sirt3","entity_type_key":"protein"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""},{"entity":{"id":"b3649eb8-9529-52ab-b2ae-38ec2ba141f3","slug":"mouse-sod2","display_name":"Mouse mitochondrial superoxide dismutase / Sod2","entity_type_key":"protein"},"role":"decreased acetylated readout","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"Apigenin plus MSU","comparator":"MSU alone","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"0.2 mg/mL MSU challenge","comparator":"MSU alone","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"monosodium-urate-crystals","display_name":"Monosodium urate crystals","entity_type_key":"small_molecule"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Apigenin plus MSU\", \"comparator\": \"MSU alone\", \"endpoint\": \"Apigenin restored SIRT3 protein abundance and decreased acetylated SOD2 in MSU-challenged mouse macrophages.\", \"effect_direction\": \"increase\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"apigenin\", \"state\": \"Apigenin plus MSU\"}, {\"entity_slug\": \"monosodium-urate-crystals\", \"state\": \"0.2 mg/mL MSU challenge\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Mouse BMDMs; 25 micromolar apigenin.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Apigenin restored SIRT3 protein abundance and decreased acetylated SOD2 in MSU-challenged mouse macrophages.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The NADase CD38 is a central regulator in gouty inflammation and a novel druggable therapeutic target. | https://pubmed.ncbi.nlm.nih.gov/38493256/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11058052/ | https://doi.org/10.1007/s00011-024-01863-y","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-gout-sirt3; Restored SIRT3 protein is not a direct binding or activity assay.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5b3312ce-b403-57d4-9a97-97a131f49ec7","evidence_kind":"source_excerpt","locator":"Lines 316-322","start_line":316,"end_line":322,"excerpt":"### api-gout-sirt3\n\nApigenin restored SIRT3 protein abundance and decreased acetylated SOD2 in MSU-challenged mouse macrophages.\nRestored SIRT3 protein is not a direct binding or activity assay.\nModel and exposure: Mouse BMDMs; 25 micromolar apigenin.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: The NADase CD38 is a central regulator in gouty inflammation and a novel druggable therapeutic target. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38493256/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11058052/ | https://doi.org/10.1007/s00011-024-01863-y | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"Mouse BMDMs; 25 micromolar apigenin.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}