{"id":"64c25e8e-a0fd-56d4-875e-235b6353b811","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-brain-glutamate","predicate":"increases_tested","statement":"One-month 20 mM oral D-aspartate increased extracellular prefrontal glutamate; acute 500 mg/kg injection did not increase striatal glutamate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"faecb477-ca22-5377-828f-a508e7856f92","mechanism_event_label":"The response depends on which brain region is measured.","subject":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"object":{"id":"cc669e31-c69c-5448-a5a3-5e524a0b6638","slug":"mouse-pfc-extracellular-glutamate","display_name":"Extracellular glutamate in mouse prefrontal cortex","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"faecb477-ca22-5377-828f-a508e7856f92","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-brain-glutamate-event","event_type":"observed_relationship","label":"The response depends on which brain region is measured.","description":"One-month 20 mM oral D-aspartate increased extracellular prefrontal glutamate; acute 500 mg/kg injection did not increase striatal glutamate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cc669e31-c69c-5448-a5a3-5e524a0b6638","slug":"mouse-pfc-extracellular-glutamate","display_name":"Extracellular glutamate in mouse prefrontal cortex","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse regional microdialysis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Different regions and routes remain explicit; not a claim of globally elevated brain glutamate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"D-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The response depends on which brain region is measured.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Olanzapine, but not clozapine, increases glutamate release in the prefrontal cortex of freely moving mice by inhibiting D-aspartate oxidase activity. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28393897/ · DOI 10.1038/srep46288","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fca3a229-d615-5e1e-ab11-7b5aee613637","evidence_kind":"source_excerpt","locator":"Lines 224-230","start_line":224,"end_line":230,"excerpt":"## d-aspartate-brain-glutamate\nThe response depends on which brain region is measured.\nOne-month 20 mM oral D-aspartate increased extracellular prefrontal glutamate; acute 500 mg/kg injection did not increase striatal glutamate.\nModel: Mouse regional microdialysis.\nLimitations: Different regions and routes remain explicit; not a claim of globally elevated brain glutamate.\nEvidence access: Primary full text\nOlanzapine, but not clozapine, increases glutamate release in the prefrontal cortex of freely moving mice by inhibiting D-aspartate oxidase activity. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28393897/ · DOI 10.1038/srep46288","model_system":"Mouse regional microdialysis.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3751103e-e256-5615-b24f-39cc76ccfb47","stable_key":"import-9a47f338-d127-5e4d-abf6-e99056833a69","title":"D-Aspartate: synthesis, clearance, neural and endocrine mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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