{"id":"64894d4c-1518-547f-bea3-47fe166686f5","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-lipdh-r-selectivity","predicate":"preferentially_reduce","statement":"The tested lipoamide dehydrogenase reduced R-lipoic acid about 28 times faster than S-lipoic acid.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0379cde4-d240-549a-ab44-63f20ee487fc","mechanism_event_label":"The two mirror-image forms were not equivalent substrates for this enzyme.","subject":{"id":"886512d6-6c63-5c64-8639-d443ee9f395b","slug":"mitochondrial-lipoamide-dehydrogenases","display_name":"Mitochondrial lipoamide dehydrogenases, species unspecified","entity_type_key":"protein_family"},"object":{"id":"8eeb8f85-a924-5122-9399-a757b83c668c","slug":"r-lipoic-acid","display_name":"Free R-alpha-lipoic acid","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"0379cde4-d240-549a-ab44-63f20ee487fc","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-lipdh-r-selectivity-event","event_type":"biochemical_relationship","label":"The two mirror-image forms were not equivalent substrates for this enzyme.","description":"The tested lipoamide dehydrogenase reduced R-lipoic acid about 28 times faster than S-lipoic acid.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b62d746-e407-580b-b077-1fc83a923cbb","slug":"s-lipoic-acid","display_name":"Free S-alpha-lipoic acid","entity_type_key":"small_molecule"},"role":"comparator","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"886512d6-6c63-5c64-8639-d443ee9f395b","slug":"mitochondrial-lipoamide-dehydrogenases","display_name":"Mitochondrial lipoamide dehydrogenases, species unspecified","entity_type_key":"protein_family"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8eeb8f85-a924-5122-9399-a757b83c668c","slug":"r-lipoic-acid","display_name":"Free R-alpha-lipoic acid","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/ala-research/8573188.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7631d83a60fe421318384db96c5b92458c21b0e3aeadaff85c7ae60223e40976\", \"start_char\": 0, \"end_char\": 809, \"text_sha256\": \"7631d83a60fe421318384db96c5b92458c21b0e3aeadaff85c7ae60223e40976\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified mitochondrial lipoamide dehydrogenase stereoselectivity assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"R/S lipoic acid and metabolites","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not equate the enzyme-family result with a specific human DLD preparation or whole-person clinical superiority.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Species not identified in indexed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The two mirror-image forms were not equivalent substrates for this enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ala-p8573188] Reduction of lipoic acid by lipoamide dehydrogenase. (1996). https://pubmed.ncbi.nlm.nih.gov/8573188/ DOI: 10.1016/0006-2952(95)02124-8","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified mitochondrial enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"395d40b1-fad9-5702-ac55-4afde1d8b493","evidence_kind":"source_excerpt","locator":"Lines 767-778","start_line":767,"end_line":778,"excerpt":"### ala-lipdh-r-selectivity\nThe tested lipoamide dehydrogenase reduced R-lipoic acid about 28 times faster than S-lipoic acid.\nCondition category: normal\nnutrient_topic: Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The two mirror-image forms were not equivalent substrates for this enzyme.\norganism: Species not identified in indexed abstract\ntissue_or_cell_type: Purified mitochondrial enzyme\nexperimental_model: Purified mitochondrial lipoamide dehydrogenase stereoselectivity assays\nlimitations: Do not equate the enzyme-family result with a specific human DLD preparation or whole-person clinical superiority.\nexposure: R/S lipoic acid and metabolites\nevidence_span: {\"source_cache\": \"artifacts/ala-research/8573188.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7631d83a60fe421318384db96c5b92458c21b0e3aeadaff85c7ae60223e40976\", \"start_char\": 0, \"end_char\": 809, \"text_sha256\": \"7631d83a60fe421318384db96c5b92458c21b0e3aeadaff85c7ae60223e40976\"}\n[ala-p8573188] Reduction of lipoic acid by lipoamide dehydrogenase. 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