{"id":"64718f02-07f6-5985-a612-8dd6a0dd7c82","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-chicken-gldc-plp","predicate":"binds-to","statement":"Purified chicken glycine decarboxylase contained approximately one PLP molecule per subunit.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"61036a50-21d6-5f58-80d9-8a735a3c0e40","mechanism_event_label":"This avian glycine-cleaving enzyme carries active B6.","subject":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"object":{"id":"3ff7e3ba-6422-52ba-9f7b-acb263a4a47e","slug":"chicken-gldc","display_name":"Chicken glycine decarboxylase","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"61036a50-21d6-5f58-80d9-8a735a3c0e40","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-chicken-gldc-plp-event","event_type":"biochemical_relationship","label":"This avian glycine-cleaving enzyme carries active B6.","description":"Purified chicken glycine decarboxylase contained approximately one PLP molecule per subunit.","status":"provisional","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"3ff7e3ba-6422-52ba-9f7b-acb263a4a47e","slug":"chicken-gldc","display_name":"Chicken glycine decarboxylase","entity_type_key":"protein"},"role":"avian enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"bound cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Purified chicken liver mitochondrial P-protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Chicken enzyme; not a direct assay of human GLDC.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Gallus gallus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This avian glycine-cleaving enzyme carries active B6.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b6-gldc-1980] The mitochondrial glycine cleavage system. Purification and properties of glycine decarboxylase from chicken liver mitochondria. (1980). https://pubmed.ncbi.nlm.nih.gov/7440562/ DOI: 10.1016/s0021-9258(19)70183-5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Chicken liver mitochondrial protein","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d2ef7815-1086-50bb-ac4b-c0ff512dcbda","evidence_kind":"source_excerpt","locator":"Lines 648-657","start_line":648,"end_line":657,"excerpt":"### b6-met-chicken-gldc-plp\nPurified chicken glycine decarboxylase contained approximately one PLP molecule per subunit.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This avian glycine-cleaving enzyme carries active B6.\norganism: Gallus gallus\ntissue_or_cell_type: Chicken liver mitochondrial protein\nexperimental_model: Purified chicken liver mitochondrial P-protein\nlimitations: Chicken enzyme; not a direct assay of human GLDC.\n[b6-gldc-1980] The mitochondrial glycine cleavage system. Purification and properties of glycine decarboxylase from chicken liver mitochondria. (1980). https://pubmed.ncbi.nlm.nih.gov/7440562/ DOI: 10.1016/s0021-9258(19)70183-5","model_system":"Purified chicken liver mitochondrial P-protein","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b6-gldc-1980] The mitochondrial glycine cleavage system. Purification and properties of glycine decarboxylase from chicken liver mitochondria. (1980). https://pubmed.ncbi.nlm.nih.gov/7440562/ DOI: 10.1016/s0021-9258(19)70183-5","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}