{"id":"646fbfb3-3776-51f5-a245-d8c01abf25d4","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-fly-compensation","predicate":"increases","statement":"Grape-leaf feeding increased HLCS protein and biotinylated carboxylases in Drosophila brummer mutants despite the extract inhibiting human HLCS in vitro.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"eae3d532-e0a5-5a15-9261-53e2092f0752","mechanism_event_label":"Compensatory expression can reverse an isolated-enzyme prediction.","subject":{"id":"b7ca85f3-f351-53ec-856f-8cdcd26fa62b","slug":"grape-leaf-extract-intervention","display_name":"Grape-leaf-extract intervention","entity_type_key":"cellular_process"},"object":{"id":"8953042a-0107-5f7e-86e2-54816897cd10","slug":"drosophila-biotinylated-carboxylases","display_name":"Biotinylated carboxylases in Drosophila brummer mutants","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"eae3d532-e0a5-5a15-9261-53e2092f0752","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-fly-compensation-event","event_type":"observed_relationship","label":"Compensatory expression can reverse an isolated-enzyme prediction.","description":"Grape-leaf feeding increased HLCS protein and biotinylated carboxylases in Drosophila brummer mutants despite the extract inhibiting human HLCS in vitro.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b7ca85f3-f351-53ec-856f-8cdcd26fa62b","slug":"grape-leaf-extract-intervention","display_name":"Grape-leaf-extract intervention","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8953042a-0107-5f7e-86e2-54816897cd10","slug":"drosophila-biotinylated-carboxylases","display_name":"Biotinylated carboxylases in Drosophila brummer mutants","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"31215c8f-3c76-5cba-bd32-a2bf7b4d3844","slug":"piceid","display_name":"Piceid / polydatin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Fly feeding; grape extract, with separate piceid body-fat experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not purified resveratrol exposure; reduced fly fat was not explained by lost carboxylase biotinylation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Resveratrol collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Compensatory expression can reverse an isolated-enzyme prediction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Resveratrol compounds inhibit human holocarboxylase synthetase and cause a lean phenotype in Drosophila melanogaster. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26303405/ · DOI 10.1016/j.jnutbio.2015.07.004","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4fe5e850-60a0-599e-8372-7966db18247e","evidence_kind":"source_excerpt","locator":"Lines 358-364","start_line":358,"end_line":364,"excerpt":"## resveratrol-fly-compensation\nCompensatory expression can reverse an isolated-enzyme prediction.\nGrape-leaf feeding increased HLCS protein and biotinylated carboxylases in Drosophila brummer mutants despite the extract inhibiting human HLCS in vitro.\nModel: Fly feeding; grape extract, with separate piceid body-fat experiments.\nLimitations: Not purified resveratrol exposure; reduced fly fat was not explained by lost carboxylase biotinylation.\nEvidence access: Primary full text\nResveratrol compounds inhibit human holocarboxylase synthetase and cause a lean phenotype in Drosophila melanogaster. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26303405/ · DOI 10.1016/j.jnutbio.2015.07.004","model_system":"Fly feeding; grape extract, with separate piceid body-fat experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2844eda9-a122-5357-ae47-cd58227c5994","stable_key":"import-52871722-4aa6-5453-a902-3e76356db327","title":"Resveratrol: metabolites, target selectivity and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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