{"id":"645b4e74-ce9b-5a8a-8949-46524fbf56ee","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:va-repro-retinyl-palmitate-rescue","predicate":"repletion_restores","statement":"Maternal retinyl-palmitate supplementation rescued SYCP3-positive meiotic cells in vitamin A-depleted Lrat-null pups.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"225506fc-5d69-5dc8-8728-b0bd355b2849","mechanism_event_label":"Replacing the missing nutrient restored early meiotic development.","subject":{"id":"62e3f5bd-503b-5ea8-b102-966aa3b7319c","slug":"retinyl-palmitate","display_name":"All-trans-retinyl palmitate","entity_type_key":"small_molecule"},"object":{"id":"155bea0b-22fa-5f4c-b0d5-ee64b94435c5","slug":"mouse-germ-cell-meiotic-initiation","display_name":"Mouse germ-cell meiotic initiation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"225506fc-5d69-5dc8-8728-b0bd355b2849","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:va-repro-retinyl-palmitate-rescue-event","event_type":"biochemical_relationship","label":"Replacing the missing nutrient restored early meiotic development.","description":"Maternal retinyl-palmitate supplementation rescued SYCP3-positive meiotic cells in vitamin A-depleted Lrat-null pups.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f7ce5851-df6d-54ed-b467-482480d26ff9","slug":"lrat","display_name":"Lecithin:retinol acyltransferase / LRAT","entity_type_key":"protein"},"role":"shared_knockout_background","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"148c57d8-c658-5769-89e8-1194cef2a782","slug":"retinol","display_name":"All-trans-retinol","entity_type_key":"small_molecule"},"role":"vitamin_a_precursor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"62e3f5bd-503b-5ea8-b102-966aa3b7319c","slug":"retinyl-palmitate","display_name":"All-trans-retinyl palmitate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"155bea0b-22fa-5f4c-b0d5-ee64b94435c5","slug":"mouse-germ-cell-meiotic-initiation","display_name":"Mouse germ-cell meiotic initiation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Dietary rescue experiment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"100 U/day maternal retinyl palmitate from P5; assessment at P10.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Rescue of early markers is not proof of adult fertility or a human dose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Replacing the missing nutrient restored early meiotic development.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[li2011] Vitamin A deficiency results in meiotic failure and accumulation of undifferentiated spermatogonia in prepubertal mouse testis. (2011). https://pubmed.ncbi.nlm.nih.gov/20881313/ DOI: 10.1095/biolreprod.110.086157","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"neonatal testis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"8e6c70c9-f89e-570c-810d-7e22d1082272","evidence_kind":"source_excerpt","locator":"Lines 278-289","start_line":278,"end_line":289,"excerpt":"### va-repro-retinyl-palmitate-rescue\nMaternal retinyl-palmitate supplementation rescued SYCP3-positive meiotic cells in vitamin A-depleted Lrat-null pups.\nCondition category: nutrient_deficiency\nnutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Replacing the missing nutrient restored early meiotic development.\norganism: Mus musculus\ntissue_or_cell_type: neonatal testis\nexperimental_model: Dietary rescue experiment\nlimitations: Rescue of early markers is not proof of adult fertility or a human dose.\nexposure: 100 U/day maternal retinyl palmitate from P5; assessment at P10.\ncross_nutrient: false\n[li2011] Vitamin A deficiency results in meiotic failure and accumulation of undifferentiated spermatogonia in prepubertal mouse testis. (2011). https://pubmed.ncbi.nlm.nih.gov/20881313/ DOI: 10.1095/biolreprod.110.086157","model_system":"Dietary rescue experiment","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [li2011] Vitamin A deficiency results in meiotic failure and accumulation of undifferentiated spermatogonia in prepubertal mouse testis. (2011). https://pubmed.ncbi.nlm.nih.gov/20881313/ DOI: 10.1095/biolreprod.110.086157","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"83131a07-353f-5301-9ce6-50754c194952","stable_key":"import-3c852f36-7951-5fd9-9013-d771a8d16d8a","title":"Vitamin A expansion: reproduction, light-driven vision and shared mechanisms (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"559cb4dc3f59519ecb0bc455dbcc73346fbf8e0c51e133f455781f51e4ed07ab","revision_id":"225fe4da-2d21-5c19-a92c-80a5f3cac8cb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}