{"id":"644caf5d-e555-5a69-9ef8-258f890c5fcb","stable_key":"research:selenoo-ampylates-glud1","predicate":"ampylates","statement":"SELENOO catalyzes AMP attachment to GLUD1.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"neutral","is_public":true,"mechanism_event_id":"4835c97e-6b0b-53b1-800f-faeed382dfb0","mechanism_event_label":"SELENOO can modify the metabolic enzyme GLUD1 with AMP.","subject":{"id":"90e4a389-1062-5489-bae1-d49f337c98f0","slug":"selenoo","display_name":"SELENOO","entity_type_key":"protein"},"object":{"id":"7a2271b6-fae8-5b08-b6ae-df7a0f96919b","slug":"glud1","display_name":"GLUD1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"4835c97e-6b0b-53b1-800f-faeed382dfb0","stable_key":"research:selenoo-ampylates-glud1","event_type":"ampylation","label":"SELENOO can modify the metabolic enzyme GLUD1 with AMP.","description":"SELENOO catalyzes AMP attachment to GLUD1.","status":"active","compartment":{"slug":"mitochondria","display_name":"Mitochondria"},"participants":[{"entity":{"id":"90e4a389-1062-5489-bae1-d49f337c98f0","slug":"selenoo","display_name":"SELENOO","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7a2271b6-fae8-5b08-b6ae-df7a0f96919b","slug":"glud1","display_name":"GLUD1","entity_type_key":"protein"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"amp-donor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"017b8a87-e258-5741-a0ec-7041d32c5c8f","slug":"ampylated-glud1","display_name":"AMPylated GLUD1","entity_type_key":"protein_state"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"experimental cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Biochemical AMPylation assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Substrate identification does not establish every tissue context.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"mammalian","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9b770534-21d3-556d-9de7-b525986b7e08","evidence_kind":"curated_literature_summary","locator":"lines 486-496","start_line":486,"end_line":496,"excerpt":"## selenoo-ampylates-glud1\n\nSELENOO can modify the metabolic enzyme GLUD1 with AMP.\n\nSELENOO catalyzes AMP attachment to GLUD1.\n\nOrganism: mammalian\nCell type: experimental cells\nExperimental model: Biochemical AMPylation assays\nLimitations: Substrate identification does not establish every tissue context.\nPrimary reference: [A repurposed AMP binding domain reveals mitochondrial protein AMPylation as a regulator of cellular metabolism](https://www.nature.com/articles/s41467-025-63014-z)","model_system":"Biochemical AMPylation assays","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"protein-modification","plain_language":"SELENOO can modify the metabolic enzyme GLUD1 with AMP.","evidence_scope":"direct_experimental","papers":[{"key":"gonzalez-2025-mitochondrial-ampylation","title":"A repurposed AMP binding domain reveals mitochondrial protein AMPylation as a regulator of cellular metabolism","url":"https://www.nature.com/articles/s41467-025-63014-z","doi":"10.1038/s41467-025-63014-z","year":2025,"model":"Biochemical assays and mammalian cells","summary":"Maps mitochondrial AMPylation substrates and tests inhibition of GLUD1 activity."}]}}