{"id":"63ff6add-e339-5b3d-8d5f-783b189cf7f7","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-keratinocyte-inflammation","predicate":"decreases_in_recorded_comparison","statement":"Apigenin reduced LPS-associated TLR4/Akt/mTOR/NF-kappaB and stress-kinase signaling readouts in human keratinocyte experiments.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"59412781-00fc-5944-bc46-d95f3a63754d","mechanism_event_label":"Apigenin reduced LPS-associated TLR4/Akt/mTOR/NF-kappaB and stress-kinase signaling readouts in human keratinocyte experiments.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"9498cfd1-66c0-5718-a8af-df5780cd5eec","slug":"api-human-keratinocyte-inflammatory-signaling","display_name":"Inflammatory signaling in apigenin-treated human keratinocytes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"59412781-00fc-5944-bc46-d95f3a63754d","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-keratinocyte-inflammation-event","event_type":"experimental_observation","label":"Apigenin reduced LPS-associated TLR4/Akt/mTOR/NF-kappaB and stress-kinase signaling readouts in human keratinocyte experiments.","description":"### api-keratinocyte-inflammation\n\nApigenin reduced LPS-associated TLR4/Akt/mTOR/NF-kappaB and stress-kinase signaling readouts in human keratinocyte experiments.\nPathway readouts are not evidence that each listed protein directly binds apigenin.\nModel and exposure: HEK001 and primary human keratinocytes; accessed primary abstract.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Apigenin reduces the Toll-like receptor-4-dependent activation of NF-κB by suppressing the Akt, mTOR, JNK, and p38-MAPK. | 2018 | https://pubmed.ncbi.nlm.nih.gov/29264665/ | https://doi.org/10.1007/s00210-017-1454-4 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Apigenin during LPS challenge","sequence_order":0,"notes":""},{"entity":{"id":"9498cfd1-66c0-5718-a8af-df5780cd5eec","slug":"api-human-keratinocyte-inflammatory-signaling","display_name":"Inflammatory signaling in apigenin-treated human keratinocytes","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"Apigenin during LPS challenge","comparator":"LPS alone","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"LPS challenge","comparator":"LPS alone","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"lipopolysaccharide","display_name":"Lipopolysaccharide","entity_type_key":"chemical_species"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Apigenin during LPS challenge\", \"comparator\": \"LPS alone\", \"endpoint\": \"Apigenin reduced LPS-associated TLR4/Akt/mTOR/NF-kappaB and stress-kinase signaling readouts in human keratinocyte experiments.\", \"effect_direction\": \"decrease\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"apigenin\", \"state\": \"Apigenin during LPS challenge\"}, {\"entity_slug\": \"lipopolysaccharide\", \"state\": \"LPS challenge\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"HEK001 and primary human keratinocytes; accessed primary abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Apigenin reduced LPS-associated TLR4/Akt/mTOR/NF-kappaB and stress-kinase signaling readouts in human keratinocyte experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Apigenin reduces the Toll-like receptor-4-dependent activation of NF-κB by suppressing the Akt, mTOR, JNK, and p38-MAPK. | https://pubmed.ncbi.nlm.nih.gov/29264665/ | https://doi.org/10.1007/s00210-017-1454-4","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-keratinocyte-inflammation; Pathway readouts are not evidence that each listed protein directly binds apigenin.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"842099d4-cb6e-5b83-aca1-b5aeab61c2c5","evidence_kind":"source_excerpt","locator":"Lines 768-774","start_line":768,"end_line":774,"excerpt":"### api-keratinocyte-inflammation\n\nApigenin reduced LPS-associated TLR4/Akt/mTOR/NF-kappaB and stress-kinase signaling readouts in human keratinocyte experiments.\nPathway readouts are not evidence that each listed protein directly binds apigenin.\nModel and exposure: HEK001 and primary human keratinocytes; accessed primary abstract.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Apigenin reduces the Toll-like receptor-4-dependent activation of NF-κB by suppressing the Akt, mTOR, JNK, and p38-MAPK. | 2018 | https://pubmed.ncbi.nlm.nih.gov/29264665/ | https://doi.org/10.1007/s00210-017-1454-4 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"HEK001 and primary human keratinocytes; accessed primary abstract.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}