{"id":"63cc742d-3ba8-54f4-a72f-222310a66aa7","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-human-ddo-activity","predicate":"oxidizes","statement":"Purified human DDO-1 oxidized D-aspartate; the reported Km was 2.7 mM and kcat 52.5 per second.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6fec3bf7-e42b-54d5-9ad1-35ee5bf0b5f1","mechanism_event_label":"Humans have an enzyme that breaks down D-aspartate.","subject":{"id":"17871951-099f-5a48-832e-ff9cb1c65755","slug":"ddo","display_name":"Human D-aspartate oxidase / DDO","entity_type_key":"protein"},"object":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"6fec3bf7-e42b-54d5-9ad1-35ee5bf0b5f1","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-human-ddo-activity-event","event_type":"observed_relationship","label":"Humans have an enzyme that breaks down D-aspartate.","description":"Purified human DDO-1 oxidized D-aspartate; the reported Km was 2.7 mM and kcat 52.5 per second.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"17871951-099f-5a48-832e-ff9cb1c65755","slug":"ddo","display_name":"Human D-aspartate oxidase / DDO","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e2cd7179-f218-54e8-9ce9-7a836ae35fac","slug":"fad","display_name":"FAD","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human 341-residue DDO-1.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Assay kinetic constants are not clinical plasma targets; do not assign these activities to the alternatively spliced deletion isoform.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"D-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Humans have an enzyme that breaks down D-aspartate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Structural and functional characterization of the human brain D-aspartate oxidase. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9163533/ · DOI 10.1093/oxfordjournals.jbchem.a021655","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a8e38f5e-e220-5f30-a487-382749c21e41","evidence_kind":"source_excerpt","locator":"Lines 72-78","start_line":72,"end_line":78,"excerpt":"## d-aspartate-human-ddo-activity\nHumans have an enzyme that breaks down D-aspartate.\nPurified human DDO-1 oxidized D-aspartate; the reported Km was 2.7 mM and kcat 52.5 per second.\nModel: Recombinant human 341-residue DDO-1.\nLimitations: Assay kinetic constants are not clinical plasma targets; do not assign these activities to the alternatively spliced deletion isoform.\nEvidence access: Primary abstract\nStructural and functional characterization of the human brain D-aspartate oxidase. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9163533/ · DOI 10.1093/oxfordjournals.jbchem.a021655","model_system":"Recombinant human 341-residue DDO-1.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3751103e-e256-5615-b24f-39cc76ccfb47","stable_key":"import-9a47f338-d127-5e4d-abf6-e99056833a69","title":"D-Aspartate: synthesis, clearance, neural and endocrine mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"0a86a1008edb8a69dcc79409e76e3fd5ce59b0747be8cae8f21e43f871e76248","revision_id":"a1de4b66-263d-5452-8ed8-df853afba030","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}