{"id":"63aa0b07-47b5-583c-9a27-c6cba918669d","stable_key":"561b2b40-5b59-548f-a749-f357f7e2304c:monosodium-glutamate-calhm3-channel","predicate":"forms_functional_channel_with","statement":"CALHM3 coassembly with CALHM1 conferred rapid voltage-dependent ATP-release-channel gating; the study connected this complex to type II taste-cell physiology.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0c10740f-f15d-5518-b53f-b3e037a4e21b","mechanism_event_label":"Two separately stored subunits form the output channel.","subject":{"id":"b1a0c8c2-97d1-5829-98d4-70ee89110760","slug":"mouse-calhm3","display_name":"Mouse CALHM3 / Calhm3","entity_type_key":"protein"},"object":{"id":"e6024a40-c127-5833-9faf-9aab86a5b37f","slug":"mouse-calhm1","display_name":"Mouse CALHM1 / Calhm1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"0c10740f-f15d-5518-b53f-b3e037a4e21b","stable_key":"561b2b40-5b59-548f-a749-f357f7e2304c:monosodium-glutamate-calhm3-channel-event","event_type":"observed_relationship","label":"Two separately stored subunits form the output channel.","description":"CALHM3 coassembly with CALHM1 conferred rapid voltage-dependent ATP-release-channel gating; the study connected this complex to type II taste-cell physiology.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b1a0c8c2-97d1-5829-98d4-70ee89110760","slug":"mouse-calhm3","display_name":"Mouse CALHM3 / Calhm3","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e6024a40-c127-5833-9faf-9aab86a5b37f","slug":"mouse-calhm1","display_name":"Mouse CALHM1 / Calhm1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2952387b-d69b-5530-a976-a875ad008226","slug":"monosodium-glutamate","display_name":"Monosodium L-glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e5756b83-24e0-5ce2-bbd6-fe857f152ffc","slug":"mouse-calhm1-calhm3","display_name":"Mouse CALHM1/CALHM3 ATP-release channel","entity_type_key":"protein_complex"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Heterologous channel experiments and mouse taste-cell physiology.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Functional coassembly is recorded without treating the historical proposed stoichiometry as a universal structural assignment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Monosodium Glutamate (MSG) collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"monosodium-glutamate","display_name":"Monosodium L-glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Two separately stored subunits form the output channel.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"CALHM3 Is Essential for Rapid Ion Channel-Mediated Purinergic Neurotransmission of GPCR-Mediated Tastes. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29681531/ · DOI 10.1016/j.neuron.2018.03.043","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"17ea220b-f2ed-54e4-92b4-42ce5814db27","evidence_kind":"source_excerpt","locator":"Lines 122-128","start_line":122,"end_line":128,"excerpt":"## monosodium-glutamate-calhm3-channel\nTwo separately stored subunits form the output channel.\nCALHM3 coassembly with CALHM1 conferred rapid voltage-dependent ATP-release-channel gating; the study connected this complex to type II taste-cell physiology.\nModel: Heterologous channel experiments and mouse taste-cell physiology.\nLimitations: Functional coassembly is recorded without treating the historical proposed stoichiometry as a universal structural assignment.\nEvidence access: Primary abstract\nCALHM3 Is Essential for Rapid Ion Channel-Mediated Purinergic Neurotransmission of GPCR-Mediated Tastes. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29681531/ · DOI 10.1016/j.neuron.2018.03.043","model_system":"Heterologous channel experiments and mouse taste-cell physiology.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"90a81fe5-8622-52f6-b4db-04899206c2b0","stable_key":"import-561b2b40-5b59-548f-a749-f357f7e2304c","title":"Monosodium Glutamate (MSG): taste, gut sensing, exposure and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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