{"id":"6354c13a-6686-5f60-8aee-75274f24647a","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-podocyte-p27-loss","predicate":"silencing_reduces_protection","statement":"Cdkn1b interference reduced fisetin protection against high-glucose injury in mouse podocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"299f2a2c-cd40-50fa-8cf8-57067494c53d","mechanism_event_label":"Disrupting the regulator weakened protection.","subject":{"id":"bffebe7d-585f-5975-bab6-49c744409f5e","slug":"mouse-cdkn1b","display_name":"Mouse p27 / Cdkn1b","entity_type_key":"protein"},"object":{"id":"f99b6476-68ce-5d29-abfb-9a7e0193d9d9","slug":"mouse-high-glucose-podocyte-injury","display_name":"High-glucose injury in mouse podocytes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"299f2a2c-cd40-50fa-8cf8-57067494c53d","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-podocyte-p27-loss-event","event_type":"observed_relationship","label":"Disrupting the regulator weakened protection.","description":"Cdkn1b interference reduced fisetin protection against high-glucose injury in mouse podocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bffebe7d-585f-5975-bab6-49c744409f5e","slug":"mouse-cdkn1b","display_name":"Mouse p27 / Cdkn1b","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f99b6476-68ce-5d29-abfb-9a7e0193d9d9","slug":"mouse-high-glucose-podocyte-injury","display_name":"High-glucose injury in mouse podocytes","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse podocyte gene-interference experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A pathway dependency, not evidence of a nutritional deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Disrupting the regulator weakened protection.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Fisetin Attenuates Diabetic Nephropathy-Induced Podocyte Injury by Inhibiting NLRP3 Inflammasome. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35126159/ · DOI 10.3389/fphar.2022.783706","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"12fe845e-39ce-54cd-a597-538d6013958e","evidence_kind":"source_excerpt","locator":"Lines 408-414","start_line":408,"end_line":414,"excerpt":"## fisetin-podocyte-p27-loss\nDisrupting the regulator weakened protection.\nCdkn1b interference reduced fisetin protection against high-glucose injury in mouse podocytes.\nModel: Mouse podocyte gene-interference experiment.\nLimitations: A pathway dependency, not evidence of a nutritional deficiency.\nEvidence access: Primary full text\nFisetin Attenuates Diabetic Nephropathy-Induced Podocyte Injury by Inhibiting NLRP3 Inflammasome. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35126159/ · DOI 10.3389/fphar.2022.783706","model_system":"Mouse podocyte gene-interference experiment.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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