{"id":"6312a0da-4d12-52dc-b990-3310249afecc","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-aldehyde-no","predicate":"contributes_to","statement":"Adding diamine oxidase or inhibiting aldehyde dehydrogenase increased agmatine-associated suppression of inducible NO generation; aldehyde removal or trapping attenuated it.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"34729b0b-568a-531a-bd7e-557b088536e1","mechanism_event_label":"How a compound is metabolized can change its signaling effect.","subject":{"id":"9688510b-6566-58c2-adda-8b58179ca8ae","slug":"4-guanidinobutyraldehyde","display_name":"4-Guanidinobutyraldehyde / agmatine aldehyde","entity_type_key":"small_molecule"},"object":{"id":"7efa5250-6241-51bc-bf3d-fd76d5d09150","slug":"cytokine-culture-agmatine-no-suppression","display_name":"Agmatine-associated inducible NO suppression in cytokine-stimulated cultures","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"34729b0b-568a-531a-bd7e-557b088536e1","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-aldehyde-no-event","event_type":"observed_relationship","label":"How a compound is metabolized can change its signaling effect.","description":"Adding diamine oxidase or inhibiting aldehyde dehydrogenase increased agmatine-associated suppression of inducible NO generation; aldehyde removal or trapping attenuated it.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9688510b-6566-58c2-adda-8b58179ca8ae","slug":"4-guanidinobutyraldehyde","display_name":"4-Guanidinobutyraldehyde / agmatine aldehyde","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7efa5250-6241-51bc-bf3d-fd76d5d09150","slug":"cytokine-culture-agmatine-no-suppression","display_name":"Agmatine-associated inducible NO suppression in cytokine-stimulated cultures","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"508e25ee-c4f9-53ac-9b9b-3b122a90c6d9","slug":"4-guanidinobutyrate","display_name":"4-Guanidinobutyrate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cytokine-stimulated cell preparations; enzyme additions, inhibitors and aldehyde trapping.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The accessed abstract does not fully specify each cell species, dose or enzyme isoform. It supports aldehyde involvement rather than a direct binding target.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"How a compound is metabolized can change its signaling effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Suppression of inducible nitric oxide generation by agmatine aldehyde: beneficial effects in sepsis. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11473357/ · DOI 10.1002/jcp.1119","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b4fae92e-e07b-51bc-a59e-b29b0179c4d1","evidence_kind":"source_excerpt","locator":"Lines 108-114","start_line":108,"end_line":114,"excerpt":"## agmatine-sulfate-aldehyde-no\nHow a compound is metabolized can change its signaling effect.\nAdding diamine oxidase or inhibiting aldehyde dehydrogenase increased agmatine-associated suppression of inducible NO generation; aldehyde removal or trapping attenuated it.\nModel: Cytokine-stimulated cell preparations; enzyme additions, inhibitors and aldehyde trapping.\nLimitations: The accessed abstract does not fully specify each cell species, dose or enzyme isoform. It supports aldehyde involvement rather than a direct binding target.\nEvidence access: Primary abstract\nSuppression of inducible nitric oxide generation by agmatine aldehyde: beneficial effects in sepsis. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11473357/ · DOI 10.1002/jcp.1119","model_system":"Cytokine-stimulated cell preparations; enzyme additions, inhibitors and aldehyde trapping.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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