{"id":"62e9920a-b78e-5ae2-93c2-a38c57dd0395","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-deiodination","predicate":"decreases_in_recorded_comparison","statement":"An older rat-liver microsomal study identified RA among inhibitors of iodothyronine deiodination.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"a0d85605-b826-5c0d-9bd4-7ff04897dbab","mechanism_event_label":"An older rat-liver microsomal study identified RA among inhibitors of iodothyronine deiodination.","subject":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"object":{"id":"de2bd0a4-622a-5cb2-b31b-f265d621161d","slug":"rat-dio1","display_name":"Rat type 1 iodothyronine deiodinase","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"a0d85605-b826-5c0d-9bd4-7ff04897dbab","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-deiodination-event","event_type":"experimental_observation","label":"An older rat-liver microsomal study identified RA among inhibitors of iodothyronine deiodination.","description":"### ra-deiodination\n\nAn older rat-liver microsomal study identified RA among inhibitors of iodothyronine deiodination.\nThe assay predates modern isoform classification. It does not establish direct molecular binding to DIO1 or dietary iodine/selenium depletion.\nModel and exposure: 1984 microsomal preparation; dose-dependent in-vitro inhibition.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Antihormonal effects of plant extracts: iodothyronine deiodinase of rat liver is inhibited by extracts and secondary metabolites of plants. | 1984 | https://pubmed.ncbi.nlm.nih.gov/6724503/ | https://doi.org/10.1055/s-2007-1014739 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"RA in rat microsomal assay","sequence_order":0,"notes":""},{"entity":{"id":"de2bd0a4-622a-5cb2-b31b-f265d621161d","slug":"rat-dio1","display_name":"Rat type 1 iodothyronine deiodinase","entity_type_key":"protein"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"f2b25f38-ec94-57d0-a46d-dd0fee45f01e","slug":"thyroxine","display_name":"T4","entity_type_key":"small_molecule"},"role":"assay substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"559f37d2-ee0a-589b-8689-fa80ca9ae653","slug":"triiodothyronine","display_name":"T3","entity_type_key":"small_molecule"},"role":"deiodination product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"RA in rat microsomal assay\", \"comparator\": \"No inhibitor\", \"endpoint\": \"An older rat-liver microsomal study identified RA among inhibitors of iodothyronine deiodination.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"1984 microsomal preparation; dose-dependent in-vitro inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Rosmarinic acid collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An older rat-liver microsomal study identified RA among inhibitors of iodothyronine deiodination.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Antihormonal effects of plant extracts: iodothyronine deiodinase of rat liver is inhibited by extracts and secondary metabolites of plants. | https://pubmed.ncbi.nlm.nih.gov/6724503/ | https://doi.org/10.1055/s-2007-1014739","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference ra-deiodination; The assay predates modern isoform classification. It does not establish direct molecular binding to DIO1 or dietary iodine/selenium depletion.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ae50d407-a584-5278-96f2-35a89bc03fe6","evidence_kind":"source_excerpt","locator":"Lines 518-524","start_line":518,"end_line":524,"excerpt":"### ra-deiodination\n\nAn older rat-liver microsomal study identified RA among inhibitors of iodothyronine deiodination.\nThe assay predates modern isoform classification. It does not establish direct molecular binding to DIO1 or dietary iodine/selenium depletion.\nModel and exposure: 1984 microsomal preparation; dose-dependent in-vitro inhibition.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Antihormonal effects of plant extracts: iodothyronine deiodinase of rat liver is inhibited by extracts and secondary metabolites of plants. | 1984 | https://pubmed.ncbi.nlm.nih.gov/6724503/ | https://doi.org/10.1055/s-2007-1014739 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"1984 microsomal preparation; dose-dependent in-vitro inhibition.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"606cff22-a6ed-59cd-a84d-87f44c59dc25","stable_key":"import-ca8e9e6a-6953-51ec-b712-2b06398a416d","title":"Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"684b4eab1dc2c0057f679109770af0de9340af5cd933377b590298cf8a752956","revision_id":"4028d9c5-fb17-54c5-b72c-c6d21678a832","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}