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This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A second acetyl-CoA-producing route could compensate when enough acetate was supplied.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-met-chromatin2009] ATP-citrate lyase links cellular metabolism to histone acetylation. (2009). https://pubmed.ncbi.nlm.nih.gov/19461003/ DOI: 10.1126/science.1164097","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HCT116 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"923e3917-cd74-501f-aa28-51c4071f3ad1","evidence_kind":"source_excerpt","locator":"Lines 1005-1017","start_line":1005,"end_line":1017,"excerpt":"### b5-met-acetate-acss2-histone-rescue\nExtracellular acetate restored histone acetylation in ACLY-silenced HCT116 cells in a dose-dependent manner that required AceCS1, now named ACSS2.\nCondition category: machinery_impairment\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A second acetyl-CoA-producing route could compensate when enough acetate was supplied.\norganism: Homo sapiens\ntissue_or_cell_type: HCT116 cells\nexperimental_model: ACLY and/or AceCS1 siRNA with acetate supplementation\nlimitations: The authors identify this acetate supply as supraphysiologic; it is not a dietary recommendation and does not show rescue of an absent CoA pool. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.\nexposure: 0, 1 or 5 mM acetate for 24 hours before lysis after siRNA treatment.\ncross_nutrient: false\nevidence_location: BioC text lines 11, 51; Fig. 2A–B\n[b5-met-chromatin2009] ATP-citrate lyase links cellular metabolism to histone acetylation. (2009). https://pubmed.ncbi.nlm.nih.gov/19461003/ DOI: 10.1126/science.1164097","model_system":"ACLY and/or AceCS1 siRNA with acetate supplementation","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-met-chromatin2009] ATP-citrate lyase links cellular metabolism to histone acetylation. 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