{"id":"62a3f49c-dd5e-5ce0-8c0b-13f0d887eb17","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-rat-cysteine-metabolism","predicate":"forms_detected_metabolite","statement":"Rat cucurbitacin B metabolism profiling detected a cysteine conjugate among phase-II products; 13 metabolites were identified and seven confirmed in the overall study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"31089529-31f7-5f27-836f-3ae0feec731c","mechanism_event_label":"Cysteine also appears in a measured metabolic product.","subject":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"object":{"id":"5c1c8ce6-cd86-5217-bb96-bc4665fc1730","slug":"cucurbitacin-b-cysteine-conjugate","display_name":"Cucurbitacin B cysteine conjugate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"31089529-31f7-5f27-836f-3ae0feec731c","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-rat-cysteine-metabolism-event","event_type":"observed_relationship","label":"Cysteine also appears in a measured metabolic product.","description":"Rat cucurbitacin B metabolism profiling detected a cysteine conjugate among phase-II products; 13 metabolites were identified and seven confirmed in the overall study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5c1c8ce6-cd86-5217-bb96-bc4665fc1730","slug":"cucurbitacin-b-cysteine-conjugate","display_name":"Cucurbitacin B cysteine conjugate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat LC-MS metabolism study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not all structures had identical confirmation strength; this record does not infer human rates.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Cysteine also appears in a measured metabolic product.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolic fate of the natural anticancer agent cucurbitacin B: an LC-MS/MS-enabled profiling of its major phase I and II conjugates in vivo. · 2024 · https://pubmed.ncbi.nlm.nih.gov/39441433/ · DOI 10.1007/s00216-024-05608-y","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0a7af21f-162b-5a3b-8076-22289ce457ec","evidence_kind":"source_excerpt","locator":"Lines 364-370","start_line":364,"end_line":370,"excerpt":"## cucurbitacin-b-rat-cysteine-metabolism\nCysteine also appears in a measured metabolic product.\nRat cucurbitacin B metabolism profiling detected a cysteine conjugate among phase-II products; 13 metabolites were identified and seven confirmed in the overall study.\nModel: Rat LC-MS metabolism study.\nLimitations: Not all structures had identical confirmation strength; this record does not infer human rates.\nEvidence access: Primary abstract\nMetabolic fate of the natural anticancer agent cucurbitacin B: an LC-MS/MS-enabled profiling of its major phase I and II conjugates in vivo. · 2024 · https://pubmed.ncbi.nlm.nih.gov/39441433/ · DOI 10.1007/s00216-024-05608-y","model_system":"Rat LC-MS metabolism study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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