{"id":"62684cd8-bb09-5122-9972-da6decb824fb","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-casr-cck","predicate":"stimulates_via_casr","statement":"L-phenylalanine stimulated CaSR-dependent calcium signaling and CCK release in primary mouse duodenal I cells; CCK responses were greater at 2.5 than at 1.26 mM extracellular calcium.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"49bf72f8-3c7e-52eb-bfe8-d78c6ad7b6c7","mechanism_event_label":"Gut amino-acid sensing can depend on both the amino acid and calcium.","subject":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"object":{"id":"b9a33255-4b95-5668-b2e9-daf129f66f1a","slug":"mouse-icell-cck-secretion","display_name":"Mouse primary intestinal I-cell CCK secretion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"49bf72f8-3c7e-52eb-bfe8-d78c6ad7b6c7","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-casr-cck-event","event_type":"observed_relationship","label":"Gut amino-acid sensing can depend on both the amino acid and calcium.","description":"L-phenylalanine stimulated CaSR-dependent calcium signaling and CCK release in primary mouse duodenal I cells; CCK responses were greater at 2.5 than at 1.26 mM extracellular calcium.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b9a33255-4b95-5668-b2e9-daf129f66f1a","slug":"mouse-icell-cck-secretion","display_name":"Mouse primary intestinal I-cell CCK secretion","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"18071fe3-a86f-5dac-b440-1e2e59d22f24","slug":"mouse-casr","display_name":"Mouse calcium-sensing receptor / Casr","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary mouse I cells; stereoselective L- versus D-phenylalanine responses.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell assay responses do not establish that calcium supplements enhance satiety in humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Phenylalanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Gut amino-acid sensing can depend on both the amino acid and calcium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The extracellular calcium-sensing receptor is required for cholecystokinin secretion in response to L-phenylalanine in acutely isolated intestinal I cells. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21252045/ · DOI 10.1152/ajpgi.00342.2010","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"718c0c69-8039-5a07-bebe-330cf755502a","evidence_kind":"source_excerpt","locator":"Lines 278-284","start_line":278,"end_line":284,"excerpt":"## l-phenylalanine-casr-cck\nGut amino-acid sensing can depend on both the amino acid and calcium.\nL-phenylalanine stimulated CaSR-dependent calcium signaling and CCK release in primary mouse duodenal I cells; CCK responses were greater at 2.5 than at 1.26 mM extracellular calcium.\nModel: Primary mouse I cells; stereoselective L- versus D-phenylalanine responses.\nLimitations: Cell assay responses do not establish that calcium supplements enhance satiety in humans.\nEvidence access: Primary abstract\nThe extracellular calcium-sensing receptor is required for cholecystokinin secretion in response to L-phenylalanine in acutely isolated intestinal I cells. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21252045/ · DOI 10.1152/ajpgi.00342.2010","model_system":"Primary mouse I cells; stereoselective L- versus D-phenylalanine responses.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54de7c56-1e60-5d7b-8ef9-852c58ae92ba","stable_key":"import-56f1d914-e7da-595a-af69-c217b2b47407","title":"L-Phenylalanine: transport, protein synthesis, cofactor recycling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"4d53adcd4acc7a9aecf44ccb2623ec540c9d221a0f7c00002585870b39b8ffb8","revision_id":"fd182a81-4ab7-559a-9c77-fb4cf72982ef","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}