{"id":"6267eb8b-6a9d-5962-9177-753d9394e40a","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:fol-depletion-lymphocyte-methylation","predicate":"depletion_lowered_measured_dna_methylation","statement":"Lymphocyte DNA hypomethylation developed during the depletion periods and reversed during folate repletion of 286–516 micrograms/day.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"ef120367-0b4c-5ccc-b576-72fc0623b5f6","mechanism_event_label":"The methylation readout changed with folate intake in these blood cells.","subject":{"id":"e176a266-711e-5469-89da-3fb2d3f244ad","slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"},"object":{"id":"83814e45-eef9-508d-9d1a-0281ffd2c46c","slug":"lymphocyte-genomic-dna-methylation","display_name":"Lymphocyte genomic DNA methylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ef120367-0b4c-5ccc-b576-72fc0623b5f6","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:fol-depletion-lymphocyte-methylation-event","event_type":"observed_intervention","label":"The methylation readout changed with folate intake in these blood cells.","description":"Lymphocyte DNA hypomethylation developed during the depletion periods and reversed during folate repletion of 286–516 micrograms/day.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e176a266-711e-5469-89da-3fb2d3f244ad","slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"83814e45-eef9-508d-9d1a-0281ffd2c46c","slug":"lymphocyte-genomic-dna-methylation","display_name":"Lymphocyte genomic DNA methylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"Eight healthy postmenopausal women aged 49–63 in a metabolic unit, sequential depletion/repletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Five weeks 56 micrograms/day folate, four weeks 111, then three weeks 286–516 using folic acid added to a low-folate diet.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No specific oncogene activation, tumor formation or whole-body methylation failure was measured.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The methylation readout changed with folate intake in these blood cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[fol-jacob1998] Moderate folate depletion increases plasma homocysteine and decreases lymphocyte DNA methylation in postmenopausal women (1998). https://pubmed.ncbi.nlm.nih.gov/9649607/ DOI: 10.1093/jn/128.7.1204","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Human lymphocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"2e3d7650-9cd8-5709-8ed8-774048903374","evidence_kind":"source_excerpt","locator":"Lines 1421-1431","start_line":1421,"end_line":1431,"excerpt":"### fol-depletion-lymphocyte-methylation\nLymphocyte DNA hypomethylation developed during the depletion periods and reversed during folate repletion of 286–516 micrograms/day.\nCondition category: nutrient_deficiency\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The methylation readout changed with folate intake in these blood cells.\norganism: Homo sapiens\ntissue_or_cell_type: Human lymphocytes\nexperimental_model: Eight healthy postmenopausal women aged 49–63 in a metabolic unit, sequential depletion/repletion.\nlimitations: No specific oncogene activation, tumor formation or whole-body methylation failure was measured.\nexposure: Five weeks 56 micrograms/day folate, four weeks 111, then three weeks 286–516 using folic acid added to a low-folate diet.\n[fol-jacob1998] Moderate folate depletion increases plasma homocysteine and decreases lymphocyte DNA methylation in postmenopausal women (1998). https://pubmed.ncbi.nlm.nih.gov/9649607/ DOI: 10.1093/jn/128.7.1204","model_system":"Eight healthy postmenopausal women aged 49–63 in a metabolic unit, sequential depletion/repletion.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [fol-jacob1998] Moderate folate depletion increases plasma homocysteine and decreases lymphocyte DNA methylation in postmenopausal women (1998). https://pubmed.ncbi.nlm.nih.gov/9649607/ DOI: 10.1093/jn/128.7.1204","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}