{"id":"6213e83d-ecf8-5f3a-8f7b-f95997cb553a","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-aqp2-down","predicate":"reduces","statement":"Chronic lithium reduced AQP2 in cortex/outer medulla and inner medulla of control mice.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"f37539ee-012c-5e25-965a-503d0582e04b","mechanism_event_label":"Fewer water channels can reduce water recovery from urine.","subject":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"object":{"id":"bfb2db34-570e-565f-9c48-cf2b299cd24e","slug":"mouse-aqp2","display_name":"Mouse aquaporin 2 / Aqp2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"f37539ee-012c-5e25-965a-503d0582e04b","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-aqp2-down-event","event_type":"observed_relationship","label":"Fewer water channels can reduce water recovery from urine.","description":"Chronic lithium reduced AQP2 in cortex/outer medulla and inner medulla of control mice.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bfb2db34-570e-565f-9c48-cf2b299cd24e","slug":"mouse-aqp2","display_name":"Mouse aquaporin 2 / Aqp2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e2e3902-5e26-52e3-9c85-1d7c0ace1637","slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"15f0301f-932b-56de-84cc-524eaa75c641","slug":"mouse-scnn1a","display_name":"Mouse epithelial sodium-channel alpha / Scnn1a","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse collecting-duct study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Residual inner-medullary reduction persisted in Scnn1a knockout; not every AQP2 change required this entry route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lithium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Fewer water channels can reduce water recovery from urine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"alphaENaC-mediated lithium absorption promotes nephrogenic diabetes insipidus. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21051735/ · DOI 10.1681/ASN.2010070734","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f4e79aa8-63e1-5c3a-bc6b-877bc4c4d3ba","evidence_kind":"source_excerpt","locator":"Lines 328-334","start_line":328,"end_line":334,"excerpt":"## lithium-aqp2-down\nFewer water channels can reduce water recovery from urine.\nChronic lithium reduced AQP2 in cortex/outer medulla and inner medulla of control mice.\nModel: Mouse collecting-duct study.\nLimitations: Residual inner-medullary reduction persisted in Scnn1a knockout; not every AQP2 change required this entry route.\nEvidence access: Primary abstract\nalphaENaC-mediated lithium absorption promotes nephrogenic diabetes insipidus. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21051735/ · DOI 10.1681/ASN.2010070734","model_system":"Mouse collecting-duct study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b2dd2f3c-5c4c-5a12-9a0d-91b521ffcf98","stable_key":"import-b7798a90-72a3-5454-a321-c4be88bf0cc4","title":"Lithium: metal-sensitive enzymes, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"dc67e965ba78458cf2d88d000d687641d9104354115ab16529a3a64941bd777d","revision_id":"4af127bf-9ccc-516b-9481-fafd453c2c35","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}