{"id":"621168ff-35e2-5778-af6f-0a05f1f4c018","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-d-cofilin-adduct","predicate":"covalently_modifies_tested","statement":"Incubating purified human cofilin 1 with cucurbitacin D produced covalent adducts; mass shifts were compatible with about four compound molecules per cofilin under the tested excess-compound conditions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0987a4a2-3d89-5276-ac92-0c1476168252","mechanism_event_label":"Protein cysteines can become chemical attachment sites.","subject":{"id":"13edf322-5d31-5a4e-802f-c7528fc2ef48","slug":"cucurbitacin-d","display_name":"Cucurbitacin D","entity_type_key":"small_molecule"},"object":{"id":"b3d1d81d-9166-5e06-9e8e-c5136d5f874c","slug":"human-cfl1","display_name":"Human cofilin 1 / CFL1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"0987a4a2-3d89-5276-ac92-0c1476168252","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-d-cofilin-adduct-event","event_type":"observed_relationship","label":"Protein cysteines can become chemical attachment sites.","description":"Incubating purified human cofilin 1 with cucurbitacin D produced covalent adducts; mass shifts were compatible with about four compound molecules per cofilin under the tested excess-compound conditions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"13edf322-5d31-5a4e-802f-c7528fc2ef48","slug":"cucurbitacin-d","display_name":"Cucurbitacin D","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b3d1d81d-9166-5e06-9e8e-c5136d5f874c","slug":"human-cfl1","display_name":"Human cofilin 1 / CFL1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text PMC3751690","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human cofilin 1, 5 micromolar; up to 1:100 protein:compound molar ratio, 16 hours.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Long, high-excess biochemical incubation; does not establish selectivity or occupancy after human ingestion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Protein cysteines can become chemical attachment sites.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cucurbitacin covalent bonding to cysteine thiols: the filamentous-actin severing protein Cofilin1 as an exemplary target. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23945128/ · DOI 10.1186/1478-811X-11-58","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ecac6782-b468-5ffa-87f0-519e908e5ef6","evidence_kind":"source_excerpt","locator":"Lines 12-18","start_line":12,"end_line":18,"excerpt":"## cucurbitacin-d-cofilin-adduct\nProtein cysteines can become chemical attachment sites.\nIncubating purified human cofilin 1 with cucurbitacin D produced covalent adducts; mass shifts were compatible with about four compound molecules per cofilin under the tested excess-compound conditions.\nModel: Purified human cofilin 1, 5 micromolar; up to 1:100 protein:compound molar ratio, 16 hours.\nLimitations: Long, high-excess biochemical incubation; does not establish selectivity or occupancy after human ingestion.\nEvidence access: Primary full text PMC3751690\nCucurbitacin covalent bonding to cysteine thiols: the filamentous-actin severing protein Cofilin1 as an exemplary target. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23945128/ · DOI 10.1186/1478-811X-11-58","model_system":"Purified human cofilin 1, 5 micromolar; up to 1:100 protein:compound molar ratio, 16 hours.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"5c23ec61b9cf3d2966fb081d8617e1e1f7c522e86419b8ba2dcae09590121123","revision_id":"74251a06-3ba5-506c-b428-700eca0fb432","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}