{"id":"61f70c72-bc11-5d4c-8b49-b8f4eec46fb0","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-tat-b6","predicate":"is_cofactor_of","statement":"Human TAT is PLP dependent, with spectroscopic analysis of its cofactor-linked aldimine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3f64b80d-dd5a-52b9-b5a9-4f1514f094e6","mechanism_event_label":"The active B6 form participates in tyrosine breakdown.","subject":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"object":{"id":"f4bcff80-2f4b-5295-a5fa-14e2d6720ec9","slug":"tat","display_name":"Human tyrosine aminotransferase / TAT","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"3f64b80d-dd5a-52b9-b5a9-4f1514f094e6","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-tat-b6-event","event_type":"observed_relationship","label":"The active B6 form participates in tyrosine breakdown.","description":"Human TAT is PLP dependent, with spectroscopic analysis of its cofactor-linked aldimine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f4bcff80-2f4b-5295-a5fa-14e2d6720ec9","slug":"tat","display_name":"Human tyrosine aminotransferase / TAT","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bcfef85f-831d-5439-ba51-1aef4b090441","slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"628ddc2d-e4d9-59c7-ae76-0c0cab80a6b1","slug":"2-oxoglutarate","display_name":"2-Oxoglutarate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"9e57c89c-2e87-5bc7-b2b9-29b122942fdd","slug":"4-hydroxyphenylpyruvate","display_name":"4-Hydroxyphenylpyruvate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human enzyme spectroscopy and kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Enzyme dependence does not establish dietary B6 limitation or that adding B6 treats TAT genetic deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Tyrosine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The active B6 form participates in tyrosine breakdown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The narrow substrate specificity of human tyrosine aminotransferase--the enzyme deficient in tyrosinemia type II. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16640556/ · DOI 10.1111/j.1742-4658.2006.05202.x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7eecf6d0-0485-5f3e-9ba0-54013844036a","evidence_kind":"source_excerpt","locator":"Lines 212-218","start_line":212,"end_line":218,"excerpt":"## l-tyrosine-tat-b6\nThe active B6 form participates in tyrosine breakdown.\nHuman TAT is PLP dependent, with spectroscopic analysis of its cofactor-linked aldimine.\nModel: Recombinant human enzyme spectroscopy and kinetics.\nLimitations: Enzyme dependence does not establish dietary B6 limitation or that adding B6 treats TAT genetic deficiency.\nEvidence access: Primary abstract\nThe narrow substrate specificity of human tyrosine aminotransferase--the enzyme deficient in tyrosinemia type II. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16640556/ · DOI 10.1111/j.1742-4658.2006.05202.x","model_system":"Recombinant human enzyme spectroscopy and kinetics.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"12917df2-c6e0-5b61-850f-dbff6d4b4d30","stable_key":"import-63ce713e-6aea-59f6-9896-ca30e010b2ce","title":"L-Tyrosine: catecholamines, thyroid chemistry, pigment, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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