{"id":"61f65b6e-c638-5eac-9bdb-31b3a292c0b8","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:b12-abs-cubam-association","predicate":"complexes-with","statement":"Human renal cubilin and AMN copurified and coeluted as a tightly associated complex that required denaturing conditions for separation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"b4aacd81-8e31-5c0e-8421-5bfc2425f6d3","mechanism_event_label":"Cubilin and amnionless form a stable receptor pair.","subject":{"id":"bbd594ac-365b-507f-954d-20a0df38312d","slug":"amn","display_name":"Human amnionless / AMN","entity_type_key":"protein"},"object":{"id":"7a3dab53-fbcd-58da-9942-21f1a3ed088d","slug":"cubn","display_name":"Human cubilin / CUBN","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"b4aacd81-8e31-5c0e-8421-5bfc2425f6d3","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:b12-abs-cubam-association-event","event_type":"biochemical_relationship","label":"Cubilin and amnionless form a stable receptor pair.","description":"Human renal cubilin and AMN copurified and coeluted as a tightly associated complex that required denaturing conditions for separation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b6d9b937-895d-5e0a-8cc5-0454a0b7d373","slug":"vitamin-b12","display_name":"Vitamin B12 (cobalamins)","entity_type_key":"chemical_species"},"role":"nutrient","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d67e8f26-bb42-5f02-af30-41f021cfd2d0","slug":"human-cubam","display_name":"Human cubilin-amnionless complex","entity_type_key":"protein_complex"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bbd594ac-365b-507f-954d-20a0df38312d","slug":"amn","display_name":"Human amnionless / AMN","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7a3dab53-fbcd-58da-9942-21f1a3ed088d","slug":"cubn","display_name":"Human cubilin / CUBN","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Affinity purification and gel filtration of human kidney membrane proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"IF-cobalamin affinity chromatography; nondenaturing versus denaturing separation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Native renal purification establishes association; intestinal uptake rate is not measured by this experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b12","display_name":"Vitamin B12 (cobalamins)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Cubilin and amnionless form a stable receptor pair.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[fyfe-2004-cubam] The functional cobalamin (vitamin B12)-intrinsic factor receptor is a novel complex of cubilin and amnionless. (2004). https://pubmed.ncbi.nlm.nih.gov/14576052/ DOI: 10.1182/blood-2003-08-2852","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Renal epithelial membranes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2a953918-0075-5b7f-8cc2-12d1856bb6b3","evidence_kind":"source_excerpt","locator":"Lines 218-229","start_line":218,"end_line":229,"excerpt":"### b12-abs-cubam-association\nHuman renal cubilin and AMN copurified and coeluted as a tightly associated complex that required denaturing conditions for separation.\nCondition category: normal\nnutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Cubilin and amnionless form a stable receptor pair.\norganism: Homo sapiens\ntissue_or_cell_type: Renal epithelial membranes\nexperimental_model: Affinity purification and gel filtration of human kidney membrane proteins\nlimitations: Native renal purification establishes association; intestinal uptake rate is not measured by this experiment.\nexposure: IF-cobalamin affinity chromatography; nondenaturing versus denaturing separation\ncross_nutrient: false\n[fyfe-2004-cubam] The functional cobalamin (vitamin B12)-intrinsic factor receptor is a novel complex of cubilin and amnionless. (2004). https://pubmed.ncbi.nlm.nih.gov/14576052/ DOI: 10.1182/blood-2003-08-2852","model_system":"Affinity purification and gel filtration of human kidney membrane proteins","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [fyfe-2004-cubam] The functional cobalamin (vitamin B12)-intrinsic factor receptor is a novel complex of cubilin and amnionless. (2004). https://pubmed.ncbi.nlm.nih.gov/14576052/ DOI: 10.1182/blood-2003-08-2852","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73145023-2982-5848-aff2-8d8875d6b9c5","stable_key":"import-7fc92b9e-9cbf-556e-8719-6b5244625250","title":"Vitamin B12: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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