{"id":"61f62537-3fbc-5e12-a965-17860d7049f9","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-pineal-melatonin","predicate":"inhibits_tested","statement":"D-aspartate inhibited norepinephrine-stimulated melatonin production in rat pineal preparations, with approximately 75 micromolar required for half-maximal inhibition.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"42b8f25d-f536-5603-8644-3d1626853d32","mechanism_event_label":"The compound can reduce stimulated melatonin production in this tissue model.","subject":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"object":{"id":"0f0bc1f1-d18d-5652-bc1d-98f61f7ab65c","slug":"melatonin","display_name":"Melatonin","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"42b8f25d-f536-5603-8644-3d1626853d32","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-pineal-melatonin-event","event_type":"observed_relationship","label":"The compound can reduce stimulated melatonin production in this tissue model.","description":"D-aspartate inhibited norepinephrine-stimulated melatonin production in rat pineal preparations, with approximately 75 micromolar required for half-maximal inhibition.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0f0bc1f1-d18d-5652-bc1d-98f61f7ab65c","slug":"melatonin","display_name":"Melatonin","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat pineal gland/pinealocyte incubation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not a human sleep trial or a demonstrated interaction with a melatonin supplement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"D-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The compound can reduce stimulated melatonin production in this tissue model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"D-aspartate modulates melatonin synthesis in rat pinealocytes. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9682837/ · DOI 10.1016/s0304-3940(98)00414-5","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2e5efc78-3bb6-5ab3-99ba-e3eb4bd59aa7","evidence_kind":"source_excerpt","locator":"Lines 176-182","start_line":176,"end_line":182,"excerpt":"## d-aspartate-pineal-melatonin\nThe compound can reduce stimulated melatonin production in this tissue model.\nD-aspartate inhibited norepinephrine-stimulated melatonin production in rat pineal preparations, with approximately 75 micromolar required for half-maximal inhibition.\nModel: Rat pineal gland/pinealocyte incubation.\nLimitations: Not a human sleep trial or a demonstrated interaction with a melatonin supplement.\nEvidence access: Primary abstract\nD-aspartate modulates melatonin synthesis in rat pinealocytes. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9682837/ · DOI 10.1016/s0304-3940(98)00414-5","model_system":"Rat pineal gland/pinealocyte incubation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3751103e-e256-5615-b24f-39cc76ccfb47","stable_key":"import-9a47f338-d127-5e4d-abf6-e99056833a69","title":"D-Aspartate: synthesis, clearance, neural and endocrine mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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