{"id":"61e9f860-43f6-5f62-83a1-02e2ff5b4cd7","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:i-met-human-dio2-selenoprotein","predicate":"contains","statement":"Cloning and characterization of human DIO2 cDNA identified DIO2 as a selenoprotein.","claim_class":"identity","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"21f02c69-74ee-574c-b154-07eb6c5a67ed","mechanism_event_label":"The T4-activating enzyme contains selenium as part of its protein structure.","subject":{"id":"fc93f0af-2123-58b6-bb7a-ff800479799a","slug":"dio2","display_name":"DIO2","entity_type_key":"protein"},"object":{"id":"42620d13-f86a-5e08-acea-7bd24787cbe4","slug":"protein-incorporated-selenocysteine","display_name":"Protein-incorporated selenocysteine residue","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"21f02c69-74ee-574c-b154-07eb6c5a67ed","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:i-met-human-dio2-selenoprotein-event","event_type":"biochemical_relationship","label":"The T4-activating enzyme contains selenium as part of its protein structure.","description":"Cloning and characterization of human DIO2 cDNA identified DIO2 as a selenoprotein.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fc93f0af-2123-58b6-bb7a-ff800479799a","slug":"dio2","display_name":"DIO2","entity_type_key":"protein"},"role":"selenoprotein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"42620d13-f86a-5e08-acea-7bd24787cbe4","slug":"protein-incorporated-selenocysteine","display_name":"Protein-incorporated selenocysteine residue","entity_type_key":"chemical_species"},"role":"incorporated_residue","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dc89d7e2-8f89-577b-838f-42bf5d8ec7c8","slug":"selenium","display_name":"Selenium","entity_type_key":"nutrient_element"},"role":"element_context","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f2b25f38-ec94-57d0-a46d-dd0fee45f01e","slug":"thyroxine","display_name":"T4","entity_type_key":"small_molecule"},"role":"substrate_context","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Selenium-containing DIO2 metabolizes iodine-containing T4.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_locator","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_spans","value_text":"[{\"source_document\": \"artifacts/iodine-metabolism-sources/8755651.json\", \"source_field\": \"resultList.result[0].abstractText\", \"start_char\": 0, \"end_char\": 1406}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat and human DIO2 cDNA cloning and characterization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"DIO2 sequence and functional characterization; no nutritional intervention.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Protein composition does not establish a dietary threshold, benefit from extra selenium, or species-independent regulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iodine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iodine","display_name":"Iodine","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human DIO2; study also characterized rat orthologue","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The T4-activating enzyme contains selenium as part of its protein structure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[i-met-8755651] Cloning of the mammalian type II iodothyronine deiodinase. A selenoprotein differentially expressed and regulated in human and rat brain and other tissues. (1996). https://pubmed.ncbi.nlm.nih.gov/8755651/ DOI: 10.1172/jci118806","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Human cDNA and expression characterization","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b145e42a-02b2-55d4-8e3f-84e479927fdb","evidence_kind":"source_excerpt","locator":"Lines 1114-1127","start_line":1114,"end_line":1127,"excerpt":"### i-met-human-dio2-selenoprotein\nCloning and characterization of human DIO2 cDNA identified DIO2 as a selenoprotein.\nCondition category: normal\nnutrient_topic: Iodine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The T4-activating enzyme contains selenium as part of its protein structure.\norganism: Human DIO2; study also characterized rat orthologue\ntissue_or_cell_type: Human cDNA and expression characterization\nexperimental_model: Rat and human DIO2 cDNA cloning and characterization\nlimitations: Protein composition does not establish a dietary threshold, benefit from extra selenium, or species-independent regulation.\nexposure: DIO2 sequence and functional characterization; no nutritional intervention.\ncross_nutrient: Selenium-containing DIO2 metabolizes iodine-containing T4.\nevidence_locator: Primary abstract\nevidence_spans: [{\"source_document\": \"artifacts/iodine-metabolism-sources/8755651.json\", \"source_field\": \"resultList.result[0].abstractText\", \"start_char\": 0, \"end_char\": 1406}]\n[i-met-8755651] Cloning of the mammalian type II iodothyronine deiodinase. A selenoprotein differentially expressed and regulated in human and rat brain and other tissues. (1996). https://pubmed.ncbi.nlm.nih.gov/8755651/ DOI: 10.1172/jci118806","model_system":"Rat and human DIO2 cDNA cloning and characterization","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [i-met-8755651] Cloning of the mammalian type II iodothyronine deiodinase. A selenoprotein differentially expressed and regulated in human and rat brain and other tissues. (1996). https://pubmed.ncbi.nlm.nih.gov/8755651/ DOI: 10.1172/jci118806","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"76da623d-a34a-5a5c-a942-d7571a85c486","stable_key":"import-aaa7baba-8689-56ab-ba1e-b71542bcb8e9","title":"Iodine: thyroid hormone production, deficiency, excess and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a4885eb6f58bad4a4514c958b63834619e57b10aa7f694aa97d0f65c7d36548f","revision_id":"ea8001a1-b576-5eda-a193-883c7ad59b73","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}