{"id":"61995fcf-8be6-509c-9ccc-14ea3a9b0349","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-acc1-reaction","predicate":"carboxylates","statement":"Human ACC1 uses biotin-dependent carboxylation of acetyl-CoA to generate malonyl-CoA.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"18e27fea-9f8a-5a55-8c98-0aaec080f43c","mechanism_event_label":"ACC1 makes a building block used in fatty-acid synthesis.","subject":{"id":"ae7b709a-c5e2-5814-af16-920bddacc7c7","slug":"acaca","display_name":"Human acetyl-CoA carboxylase 1 / ACACA","entity_type_key":"protein"},"object":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"18e27fea-9f8a-5a55-8c98-0aaec080f43c","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-acc1-reaction-event","event_type":"biochemical_relationship","label":"ACC1 makes a building block used in fatty-acid synthesis.","description":"Human ACC1 uses biotin-dependent carboxylation of acetyl-CoA to generate malonyl-CoA.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"tethered carrier","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"edf44f6b-7b23-5b97-a86b-c940e5812b06","slug":"bicarbonate-ion","display_name":"Bicarbonate ion","entity_type_key":"ion"},"role":"carboxyl donor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"energy substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"52c778e0-2b9e-5ce2-8056-a0ffdd726693","slug":"malonyl-coa","display_name":"Malonyl-CoA","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"role":"B5-derived component","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"ae7b709a-c5e2-5814-af16-920bddacc7c7","slug":"acaca","display_name":"Human acetyl-CoA carboxylase 1 / ACACA","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/biotin-research/39383219.fulltext.txt\", \"locator\": \"Exact primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5f7d3cd6a5865a03b8dc9c510f60249ee2c9f6516773780e5d7b4aef0a9b2db8\", \"start_char\": 0, \"end_char\": 309, \"text_sha256\": \"b094342ce091c8f919d065311ecd04ddd0c6b9b2824ba8adedd5768424a81a71\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cryo-EM of endogenous and recombinant human ACC1 filaments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Inactive substrate-containing and dephosphorylated/citrate-treated states","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Filament presence alone does not mean enzyme activation; the paper resolved both active and inactive arrangements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Biotin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"ACC1 makes a building block used in fatty-acid synthesis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b7-p39383219] Filament structures unveil the dynamic organization of human acetyl-CoA carboxylase. (2024). https://pubmed.ncbi.nlm.nih.gov/39383219/ DOI: 10.1126/sciadv.ado4880","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified human ACC1","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"806f795c-f94b-528b-a2fc-d874bc17f058","evidence_kind":"source_excerpt","locator":"Lines 637-648","start_line":637,"end_line":648,"excerpt":"### b7-acc1-reaction\nHuman ACC1 uses biotin-dependent carboxylation of acetyl-CoA to generate malonyl-CoA.\nCondition category: normal\nnutrient_topic: Biotin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: ACC1 makes a building block used in fatty-acid synthesis.\norganism: Homo sapiens\ntissue_or_cell_type: Purified human ACC1\nexperimental_model: Cryo-EM of endogenous and recombinant human ACC1 filaments\nlimitations: Filament presence alone does not mean enzyme activation; the paper resolved both active and inactive arrangements.\nexposure: Inactive substrate-containing and dephosphorylated/citrate-treated states\nevidence_span: {\"source_cache\": \"artifacts/biotin-research/39383219.fulltext.txt\", \"locator\": \"Exact primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5f7d3cd6a5865a03b8dc9c510f60249ee2c9f6516773780e5d7b4aef0a9b2db8\", \"start_char\": 0, \"end_char\": 309, \"text_sha256\": \"b094342ce091c8f919d065311ecd04ddd0c6b9b2824ba8adedd5768424a81a71\"}\n[b7-p39383219] Filament structures unveil the dynamic organization of human acetyl-CoA carboxylase. (2024). https://pubmed.ncbi.nlm.nih.gov/39383219/ DOI: 10.1126/sciadv.ado4880","model_system":"Cryo-EM of endogenous and recombinant human ACC1 filaments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b7-p39383219] Filament structures unveil the dynamic organization of human acetyl-CoA carboxylase. (2024). https://pubmed.ncbi.nlm.nih.gov/39383219/ DOI: 10.1126/sciadv.ado4880","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9608806b-adb6-5a35-b042-057147135642","stable_key":"import-08ce9896-9d1c-5bbf-b705-5bfe771091d5","title":"Biotin: carboxylases, recycling, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a05b23a45e0813ba2fcda0027e3f5d9d58b82858f8dd8598ffb7aca17e942a38","revision_id":"e0d0c2a2-e9e2-55dd-b467-41c22ba960d4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}