{"id":"6186a1b0-bc61-5901-bfd9-ad8fb3b83fd5","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-renal-enzyme","predicate":"hydrolyzes","statement":"Theanine-degrading activity copurified with rat renal phosphate-independent glutaminase activity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"1adca763-5a25-5871-84c4-70f8cb217320","mechanism_event_label":"One renal enzyme preparation broke down theanine; it must not be confused with every glutaminase.","subject":{"id":"c9179efe-d2e8-592c-970f-00890f2e0d71","slug":"rat-renal-phosphate-independent-glutaminase","display_name":"Rat renal phosphate-independent glutaminase preparation, gene identity unresolved","entity_type_key":"protein_family"},"object":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"1adca763-5a25-5871-84c4-70f8cb217320","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-renal-enzyme-event","event_type":"biochemical_relationship","label":"One renal enzyme preparation broke down theanine; it must not be confused with every glutaminase.","description":"Theanine-degrading activity copurified with rat renal phosphate-independent glutaminase activity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"hydrolysis_product","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"08bb4c21-b2ce-597c-b00e-7800d8f042c1","slug":"ethylamine","display_name":"Ethylamine","entity_type_key":"small_molecule"},"role":"hydrolysis_product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c9179efe-d2e8-592c-970f-00890f2e0d71","slug":"rat-renal-phosphate-independent-glutaminase","display_name":"Rat renal phosphate-independent glutaminase preparation, gene identity unresolved","entity_type_key":"protein_family"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/theanine-research/12595072.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"8a9ae8e6ba86ea3e06440e972c30ca28685032dc3b9cd74c2d98311715c2435e\", \"start_char\": 0, \"end_char\": 1324, \"text_sha256\": \"8a9ae8e6ba86ea3e06440e972c30ca28685032dc3b9cd74c2d98311715c2435e\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Renal enzyme purification and substrate-specificity assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Theanine and glutamine substrates; phosphate-dependent versus independent preparations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Assay-defined enzyme identity is not assigned to human GLS or GLS2. Rat tissue distribution does not prove the human kidney is the exclusive metabolic site.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Theanine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Wistar rat tissue and purified enzyme preparations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"One renal enzyme preparation broke down theanine; it must not be confused with every glutaminase.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[theanine-p12595072] Theanine, gamma-glutamylethylamide, is metabolized by renal phosphate-independent glutaminase. (2003). https://pubmed.ncbi.nlm.nih.gov/12595072/ DOI: 10.1016/s0304-4165(02)00504-4","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Kidney hydrolysis and gamma-glutamyl transfer","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c36638fa-48f1-59b9-9b62-1ffbd14c1ef5","evidence_kind":"source_excerpt","locator":"Lines 146-157","start_line":146,"end_line":157,"excerpt":"### theanine-renal-enzyme\nTheanine-degrading activity copurified with rat renal phosphate-independent glutaminase activity.\nCondition category: normal\nnutrient_topic: L-Theanine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: One renal enzyme preparation broke down theanine; it must not be confused with every glutaminase.\norganism: Wistar rat tissue and purified enzyme preparations\ntissue_or_cell_type: Kidney hydrolysis and gamma-glutamyl transfer\nexperimental_model: Renal enzyme purification and substrate-specificity assays\nlimitations: Assay-defined enzyme identity is not assigned to human GLS or GLS2. Rat tissue distribution does not prove the human kidney is the exclusive metabolic site.\nexposure: Theanine and glutamine substrates; phosphate-dependent versus independent preparations\nevidence_span: {\"source_cache\": \"artifacts/theanine-research/12595072.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"8a9ae8e6ba86ea3e06440e972c30ca28685032dc3b9cd74c2d98311715c2435e\", \"start_char\": 0, \"end_char\": 1324, \"text_sha256\": \"8a9ae8e6ba86ea3e06440e972c30ca28685032dc3b9cd74c2d98311715c2435e\"}\n[theanine-p12595072] Theanine, gamma-glutamylethylamide, is metabolized by renal phosphate-independent glutaminase. (2003). https://pubmed.ncbi.nlm.nih.gov/12595072/ DOI: 10.1016/s0304-4165(02)00504-4","model_system":"Renal enzyme purification and substrate-specificity assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [theanine-p12595072] Theanine, gamma-glutamylethylamide, is metabolized by renal phosphate-independent glutaminase. (2003). https://pubmed.ncbi.nlm.nih.gov/12595072/ DOI: 10.1016/s0304-4165(02)00504-4","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2bf3fc8-fc9a-5d50-937a-559a76f8be7d","stable_key":"import-8f12ba85-1a92-5337-a9c0-9cee9b3be90d","title":"L-Theanine: metabolism, neural signaling, nutrient connections and human outcomes (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"c78f2a1961da546cd8d3ae9131a16613aed0d1032904ca19175b0641647142c1","revision_id":"4ec64995-1e5b-5279-9102-f203cb2e29e5","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}