{"id":"61765ff5-ee8f-539e-8dce-6b47639e781e","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-osgepl1-charging","predicate":"when_deleted_reduces","statement":"OSGEPL1 deletion in HEK293T cells reduced aminoacylation of mitochondrial tRNA Thr and tRNA Lys and altered other tRNA modifications.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"323d00f6-8f02-52b0-bad4-230e396b19ae","mechanism_event_label":"Threonine-derived chemistry supports the handling of another amino acid too.","subject":{"id":"f28550d6-0143-5fe3-ae30-1e634dbd024d","slug":"osgepl1","display_name":"Human mitochondrial tRNA threonylcarbamoyltransferase / OSGEPL1","entity_type_key":"protein"},"object":{"id":"614e551c-1afc-57ec-ae23-6f54c3e6911b","slug":"human-mt-thr-lys-trna-charging","display_name":"Human mitochondrial threonine and lysine tRNA charging","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"323d00f6-8f02-52b0-bad4-230e396b19ae","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-osgepl1-charging-event","event_type":"observed_relationship","label":"Threonine-derived chemistry supports the handling of another amino acid too.","description":"OSGEPL1 deletion in HEK293T cells reduced aminoacylation of mitochondrial tRNA Thr and tRNA Lys and altered other tRNA modifications.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f28550d6-0143-5fe3-ae30-1e634dbd024d","slug":"osgepl1","display_name":"Human mitochondrial tRNA threonylcarbamoyltransferase / OSGEPL1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"614e551c-1afc-57ec-ae23-6f54c3e6911b","slug":"human-mt-thr-lys-trna-charging","display_name":"Human mitochondrial threonine and lysine tRNA charging","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5fff6664-209e-5c35-b88e-ac15c1c5fbc0","slug":"l-lysine","display_name":"L-Lysine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"5c27b149-da7e-5a90-8bd3-d4b2a42bb2ef","slug":"trna-threonylcarbamoyladenosine","display_name":"tRNA-bound N6-threonylcarbamoyladenosine / t6A37","entity_type_key":"rna"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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