{"id":"61045dd8-4877-5b62-97dc-3744b33f8190","stable_key":"bf161ddf-8b82-5d65-b86d-0b41245aac61:phlorizin-human-sglt2-identification","predicate":"inhibits","statement":"Human SGLT2 expressed in Xenopus oocytes transported glucose with a 1:1 sodium-to-glucose ratio and was phlorizin-sensitive.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"bedeadd9-5b04-5379-a869-fbc558bd7558","mechanism_event_label":"Human SGLT2 expressed in Xenopus oocytes transported glucose with a 1:1 sodium-to-glucose ratio and was phlorizin-sensitive.","subject":{"id":"3516a0ba-1f72-5e1b-bccb-7fca992f96f7","slug":"phlorizin","display_name":"Phlorizin","entity_type_key":"small_molecule"},"object":{"id":"dda53e42-9ad8-5cd6-8900-ff23e9699159","slug":"human-sglt2-sodium-glucose-cotransport","display_name":"Human SGLT2 sodium/glucose cotransport","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"bedeadd9-5b04-5379-a869-fbc558bd7558","stable_key":"bf161ddf-8b82-5d65-b86d-0b41245aac61:phlorizin-human-sglt2-identification-event","event_type":"observed_relationship","label":"Human SGLT2 expressed in Xenopus oocytes transported glucose with a 1:1 sodium-to-glucose ratio and was phlorizin-sensitive.","description":"Human SGLT2 expressed in Xenopus oocytes transported glucose with a 1:1 sodium-to-glucose ratio and was phlorizin-sensitive.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3516a0ba-1f72-5e1b-bccb-7fca992f96f7","slug":"phlorizin","display_name":"Phlorizin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dda53e42-9ad8-5cd6-8900-ff23e9699159","slug":"human-sglt2-sodium-glucose-cotransport","display_name":"Human SGLT2 sodium/glucose cotransport","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"43e6358a-13ec-5538-bae6-9135b0b26b8a","slug":"slc5a2","display_name":"Human sodium/glucose cotransporter 2 / SGLT2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Glucose or alpha-methylglucose with sodium and phlorizin","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute transport assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human SGLT2 expressed in Xenopus oocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Oocyte transport identifies function but does not define human oral phlorizin exposure or renal selectivity in vivo.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Phlorizin chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"phlorizin","display_name":"Phlorizin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human SGLT2 expressed in Xenopus oocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Human SGLT2 expressed in Xenopus oocytes transported glucose with a 1:1 sodium-to-glucose ratio and was phlorizin-sensitive.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The human kidney low affinity Na+/glucose cotransporter SGLT2. Delineation of the major renal reabsorptive mechanism for D-glucose. (1994). https://pubmed.ncbi.nlm.nih.gov/8282810/ DOI: 10.1172/JCI116972","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Radiotracer uptake and voltage-clamp current","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7a93c40f-f6fb-58c3-bdf9-2a54d9e45893","evidence_kind":"source_excerpt","locator":"Lines 11-20","start_line":11,"end_line":20,"excerpt":"## phlorizin-human-sglt2-identification\nHuman SGLT2 expressed in Xenopus oocytes transported glucose with a 1:1 sodium-to-glucose ratio and was phlorizin-sensitive.\nModel/species: Human SGLT2 expressed in Xenopus oocytes\nTissue/system: Radiotracer uptake and voltage-clamp current\nExposure: Glucose or alpha-methylglucose with sodium and phlorizin\nRoute: In vitro\nDuration: Acute transport assay\nLimits: Oocyte transport identifies function but does not define human oral phlorizin exposure or renal selectivity in vivo.\nPrimary reference: The human kidney low affinity Na+/glucose cotransporter SGLT2. Delineation of the major renal reabsorptive mechanism for D-glucose. (1994). https://pubmed.ncbi.nlm.nih.gov/8282810/ DOI: 10.1172/JCI116972\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Human SGLT2 expressed in Xenopus oocytes","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7d4bfcb9-1665-5686-8b8a-1ad17ad6a4d2","stable_key":"import-bf161ddf-8b82-5d65-b86d-0b41245aac61","title":"Phlorizin: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"13aa8f888801708147608784e74ad34d625b10fd76ea5afd542a3f9328bd40e2","revision_id":"6997ff77-8628-59b7-878c-95fed9d6a622","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}