{"id":"60fb2994-9026-5ff6-94a6-df5791fa6c1e","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-ears2-intermediate","predicate":"forms","statement":"Human mitochondrial glutamyl-tRNA synthetase attached glutamate to mitochondrial tRNA(Gln), generating the intermediate Glu-tRNA(Gln).","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7e8bdc73-d1f8-56ff-9722-f31733e15e03","mechanism_event_label":"A deliberately intermediate amino-acid attachment must be processed before translation.","subject":{"id":"5253bb50-e0e1-57a6-ab69-eceaac1a1c65","slug":"ears2","display_name":"Human mitochondrial glutamyl-tRNA synthetase / EARS2","entity_type_key":"protein"},"object":{"id":"d7bb28eb-0aef-5be7-b455-c801b311ca61","slug":"human-mt-glu-trna-gln","display_name":"Human mitochondrial glutamyl-tRNA(Gln)","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"7e8bdc73-d1f8-56ff-9722-f31733e15e03","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-ears2-intermediate-event","event_type":"observed_relationship","label":"A deliberately intermediate amino-acid attachment must be processed before translation.","description":"Human mitochondrial glutamyl-tRNA synthetase attached glutamate to mitochondrial tRNA(Gln), generating the intermediate Glu-tRNA(Gln).","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5253bb50-e0e1-57a6-ab69-eceaac1a1c65","slug":"ears2","display_name":"Human mitochondrial glutamyl-tRNA synthetase / EARS2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d7bb28eb-0aef-5be7-b455-c801b311ca61","slug":"human-mt-glu-trna-gln","display_name":"Human mitochondrial glutamyl-tRNA(Gln)","entity_type_key":"rna"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human enzyme and mitochondrial tRNA biochemistry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This intermediate is not glutamate being correctly incorporated at glutamine codons.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A deliberately intermediate amino-acid attachment must be processed before translation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Biogenesis of glutaminyl-mt tRNAGln in human mitochondria. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19805282/ · DOI 10.1073/pnas.0907602106","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0c817fce-569c-504e-babc-1cbcc69a2b53","evidence_kind":"source_excerpt","locator":"Lines 370-376","start_line":370,"end_line":376,"excerpt":"## glutamate-ears2-intermediate\nA deliberately intermediate amino-acid attachment must be processed before translation.\nHuman mitochondrial glutamyl-tRNA synthetase attached glutamate to mitochondrial tRNA(Gln), generating the intermediate Glu-tRNA(Gln).\nModel: Recombinant human enzyme and mitochondrial tRNA biochemistry.\nLimitations: This intermediate is not glutamate being correctly incorporated at glutamine codons.\nEvidence access: Primary abstract\nBiogenesis of glutaminyl-mt tRNAGln in human mitochondria. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19805282/ · DOI 10.1073/pnas.0907602106","model_system":"Recombinant human enzyme and mitochondrial tRNA biochemistry.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c6a55239-e808-5ed7-9fa3-755a18ecb29f","stable_key":"import-b7014def-74ec-599e-8347-167e24fec1f0","title":"L-Glutamate / L-glutamic acid: carbon and nitrogen allocation, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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