{"id":"60c72728-1e8f-50e0-be9e-2337db23d8e4","stable_key":"d40a4478-67a9-5bdf-b7a2-eb7d17e24dd7:iodine-export-2016-abundance","predicate":"increases_abundance","statement":"Pendrin abundance increased after 24 hours at 1 mM NaI; lower tested concentrations and 48-hour total abundance showed no significant increase.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"68f0807b-811a-5c10-8f14-49befbb98fdb","mechanism_event_label":"Pendrin abundance increased after 24 hours at 1 mM NaI; lower tested concentrations and 48-hour total abundance showed no significant increase.","subject":{"id":"83b11ca6-52c7-5a1b-8c39-d9cdf89244e3","slug":"iodide","display_name":"Iodide ion","entity_type_key":"ion"},"object":{"id":"3af1ca2d-4042-52fd-ad99-c4a44e5cf2fe","slug":"rat-slc26a4","display_name":"Rat pendrin / Slc26a4","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"68f0807b-811a-5c10-8f14-49befbb98fdb","stable_key":"d40a4478-67a9-5bdf-b7a2-eb7d17e24dd7:iodine-export-2016-abundance-event","event_type":"observed_intervention","label":"Pendrin abundance increased after 24 hours at 1 mM NaI; lower tested concentrations and 48-hour total abundance showed no significant increase.","description":"Pendrin abundance increased after 24 hours at 1 mM NaI; lower tested concentrations and 48-hour total abundance showed no significant increase.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"83b11ca6-52c7-5a1b-8c39-d9cdf89244e3","slug":"iodide","display_name":"Iodide ion","entity_type_key":"ion"},"role":"exposure","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3af1ca2d-4042-52fd-ad99-c4a44e5cf2fe","slug":"rat-slc26a4","display_name":"Rat pendrin / Slc26a4","entity_type_key":"protein"},"role":"measured_target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Rat thyroid PCCl3 cells; 1 mM NaI unless specified.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure_category","value_text":"Experimental iodide excess; normal is the schema category outside deficiency, machinery impairment and biomarker context.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Nonpolarized cells; concurrent changes do not establish pendrin necessity, human protection, or an intake threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"measurement","value_text":"Western blot; Figure 1.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iodine","comparator":null,"unit":null,"notes":"","entity":{"slug":"iodine","display_name":"Iodine","entity_type_key":"nutrient_element"}},{"dimension":"primary_references","value_text":"Calil-Silveira et al. (2016). Iodide excess regulates its own efflux: a possible involvement of pendrin. DOI: 10.1152/ajpcell.00210.2015. https://pubmed.ncbi.nlm.nih.gov/26791486/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"72ee3b2a-a8b3-57ff-b036-4b60dd40887c","evidence_kind":"source_excerpt","locator":"Lines 9-11","start_line":9,"end_line":11,"excerpt":"## abundance\nPendrin abundance increased after 24 hours at 1 mM NaI; lower tested concentrations and 48-hour total abundance showed no significant increase.\nMeasurement: Western blot; Figure 1.","model_system":"Rat thyroid PCCl3 cells; 1 mM NaI unless specified.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact curation-document span. Primary study paraphrased; no independent replication implied.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2b88cda3-3556-5f62-b7f5-b12270e6f766","stable_key":"import-d40a4478-67a9-5bdf-b7a2-eb7d17e24dd7","title":"Iodine addendum: iodide excess and cellular export (2026-09-18)","document_type":"imported_text","citation_label":"Calil-Silveira et al. (2016). Iodide excess regulates its own efflux: a possible involvement of pendrin. DOI: 10.1152/ajpcell.00210.2015. https://pubmed.ncbi.nlm.nih.gov/26791486/","file_path":"","sha256":"329cc12a4aaa7d6ccaeca0e5c1ffa3e447ef2debd046fc2bcd9e4f3ae33a13b3","revision_id":"93964ea3-ead3-50bb-ad49-c05a425f73d8","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}