{"id":"60a893ee-41d3-59cc-9386-368deae66f69","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-rat-odc-timing","predicate":"reduces","statement":"Agmatine reduced ODC activity before antizyme rose; proteasome inhibition did not prevent the ODC effect in rat pulmonary endothelial cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"059b4180-7c9e-514a-97e5-7b14a3b49325","mechanism_event_label":"Antizyme induction did not explain every observed effect in this preparation.","subject":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"object":{"id":"9dbd40aa-cafd-5b9e-ae1f-bafc5af7f80d","slug":"rat-pulmonary-endothelial-odc-activity","display_name":"Rat pulmonary-endothelial ODC activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"059b4180-7c9e-514a-97e5-7b14a3b49325","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-rat-odc-timing-event","event_type":"observed_relationship","label":"Antizyme induction did not explain every observed effect in this preparation.","description":"Agmatine reduced ODC activity before antizyme rose; proteasome inhibition did not prevent the ODC effect in rat pulmonary endothelial cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9dbd40aa-cafd-5b9e-ae1f-bafc5af7f80d","slug":"rat-pulmonary-endothelial-odc-activity","display_name":"Rat pulmonary-endothelial ODC activity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat pulmonary artery endothelial culture; timing and proteasome interventions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The mechanism was not resolved; this is a limit on generalizing the antizyme-only model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Antizyme induction did not explain every observed effect in this preparation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Regulation of ornithine decarboxylase activity and polyamine transport by agmatine in rat pulmonary artery endothelial cells. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11160620/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d064794e-609d-59b2-8caf-955d4916cd44","evidence_kind":"source_excerpt","locator":"Lines 356-362","start_line":356,"end_line":362,"excerpt":"## agmatine-sulfate-rat-odc-timing\nAntizyme induction did not explain every observed effect in this preparation.\nAgmatine reduced ODC activity before antizyme rose; proteasome inhibition did not prevent the ODC effect in rat pulmonary endothelial cells.\nModel: Rat pulmonary artery endothelial culture; timing and proteasome interventions.\nLimitations: The mechanism was not resolved; this is a limit on generalizing the antizyme-only model.\nEvidence access: Primary abstract\nRegulation of ornithine decarboxylase activity and polyamine transport by agmatine in rat pulmonary artery endothelial cells. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11160620/","model_system":"Rat pulmonary artery endothelial culture; timing and proteasome interventions.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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