{"id":"607c65e5-94cc-5870-af2e-b9b72a6596b0","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-fish-decamer-potency","predicate":"inhibits","statement":"Decavanadate inhibited isolated Sparus aurata heart mitochondrial oxygen consumption more potently than monomeric vanadate in the reported assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"411b24fc-1d99-5eae-86e1-a096e2bc924c","mechanism_event_label":"Oligomeric form changed potency in an isolated organelle.","subject":{"id":"ab633bfc-d259-5deb-ac81-a3383f79d4bf","slug":"decavanadate","display_name":"Decavanadate(V)","entity_type_key":"chemical_species"},"object":{"id":"667e5eb4-8764-560f-819b-098e9f7fed2f","slug":"sparus-aurata-mitochondrial-respiration","display_name":"Respiration of isolated Sparus aurata heart mitochondria","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"411b24fc-1d99-5eae-86e1-a096e2bc924c","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-fish-decamer-potency-event","event_type":"observed_relationship","label":"Oligomeric form changed potency in an isolated organelle.","description":"Decavanadate inhibited isolated Sparus aurata heart mitochondrial oxygen consumption more potently than monomeric vanadate in the reported assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ab633bfc-d259-5deb-ac81-a3383f79d4bf","slug":"decavanadate","display_name":"Decavanadate(V)","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"667e5eb4-8764-560f-819b-098e9f7fed2f","slug":"sparus-aurata-mitochondrial-respiration","display_name":"Respiration of isolated Sparus aurata heart mitochondria","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Fish cardiac mitochondria; IC50 400 nM V10 versus 23 micromolar V1 as reported.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Species and concentration basis matter; compare ten V atoms per decamer before inferring a per-atom potency ratio.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Oligomeric form changed potency in an isolated organelle.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Mitochondria as a target for decavanadate toxicity in Sparus aurata heart. · 2007 · https://pubmed.ncbi.nlm.nih.gov/17420061/ · DOI 10.1016/j.aquatox.2007.03.005","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"77b7dea3-9fb6-5efb-88da-64d61a41bb97","evidence_kind":"source_excerpt","locator":"Lines 310-316","start_line":310,"end_line":316,"excerpt":"## vanadium-fish-decamer-potency\nOligomeric form changed potency in an isolated organelle.\nDecavanadate inhibited isolated Sparus aurata heart mitochondrial oxygen consumption more potently than monomeric vanadate in the reported assay.\nModel: Fish cardiac mitochondria; IC50 400 nM V10 versus 23 micromolar V1 as reported.\nLimitations: Species and concentration basis matter; compare ten V atoms per decamer before inferring a per-atom potency ratio.\nEvidence access: Primary abstract\nMitochondria as a target for decavanadate toxicity in Sparus aurata heart. · 2007 · https://pubmed.ncbi.nlm.nih.gov/17420061/ · DOI 10.1016/j.aquatox.2007.03.005","model_system":"Fish cardiac mitochondria; IC50 400 nM V10 versus 23 micromolar V1 as reported.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e4d5d0ba-529b-540f-96ed-e719d116ccfd","stable_key":"import-335be270-ea4a-5c8e-ad04-964fd22a439e","title":"Vanadium: speciation, phosphate-sensitive enzymes and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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