{"id":"607bc96d-6feb-56ef-91cd-ba6d15dbb4c7","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-estrogen-pair","predicate":"reported_relationship","statement":"Human erythrocyte COMT catalyzed 2-hydroxyestrone conversion to 2-methoxyestrone.","claim_class":"mechanistic","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"dc61792a-04dc-5ca9-9018-58f51722d341","mechanism_event_label":"Human erythrocyte COMT catalyzed 2-hydroxyestrone conversion to 2-methoxyestrone.","subject":{"id":"936e1fd4-e862-5e32-b7dc-35b946294e43","slug":"comt","display_name":"Human catechol O-methyltransferase / COMT","entity_type_key":"protein"},"object":{"id":"cc30e923-11f8-54da-a3d9-85524d57e925","slug":"2-methoxyestrone","display_name":"2-Methoxyestrone","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"dc61792a-04dc-5ca9-9018-58f51722d341","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-estrogen-pair-event","event_type":"biochemical_relationship","label":"Human erythrocyte COMT catalyzed 2-hydroxyestrone conversion to 2-methoxyestrone.","description":"### ra-estrogen-pair\n\nHuman erythrocyte COMT catalyzed 2-hydroxyestrone conversion to 2-methoxyestrone.\nReaction identity correction: COMT methylation does not turn estrone into estradiol.\nModel and exposure: Human red-cell metabolism study; no RA intervention.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Metabolism of catechol estrogen by human erythrocytes. | 1977 | https://pubmed.ncbi.nlm.nih.gov/925137/ | https://doi.org/10.1210/jcem-45-5-1120 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"936e1fd4-e862-5e32-b7dc-35b946294e43","slug":"comt","display_name":"Human catechol O-methyltransferase / COMT","entity_type_key":"protein"},"role":"catalyst","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"33177d0a-6824-516a-b6dc-026c4d7b551e","slug":"2-hydroxyestrone","display_name":"2-Hydroxyestrone","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"methyl donor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"cc30e923-11f8-54da-a3d9-85524d57e925","slug":"2-methoxyestrone","display_name":"2-Methoxyestrone","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e84829b1-0607-558e-8add-aaa3af4747e7","slug":"s-adenosylhomocysteine","display_name":"S-Adenosyl-L-homocysteine","entity_type_key":"small_molecule"},"role":"methyl-transfer coproduct","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human red-cell metabolism study; no RA intervention.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Rosmarinic acid collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Human erythrocyte COMT catalyzed 2-hydroxyestrone conversion to 2-methoxyestrone.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolism of catechol estrogen by human erythrocytes. | https://pubmed.ncbi.nlm.nih.gov/925137/ | https://doi.org/10.1210/jcem-45-5-1120","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference ra-estrogen-pair; Reaction identity correction: COMT methylation does not turn estrone into estradiol.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f4197ebf-8d4a-5bd2-84c4-f6a80d8bafae","evidence_kind":"source_excerpt","locator":"Lines 526-532","start_line":526,"end_line":532,"excerpt":"### ra-estrogen-pair\n\nHuman erythrocyte COMT catalyzed 2-hydroxyestrone conversion to 2-methoxyestrone.\nReaction identity correction: COMT methylation does not turn estrone into estradiol.\nModel and exposure: Human red-cell metabolism study; no RA intervention.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Metabolism of catechol estrogen by human erythrocytes. | 1977 | https://pubmed.ncbi.nlm.nih.gov/925137/ | https://doi.org/10.1210/jcem-45-5-1120 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"Human red-cell metabolism study; no RA intervention.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"606cff22-a6ed-59cd-a84d-87f44c59dc25","stable_key":"import-ca8e9e6a-6953-51ec-b712-2b06398a416d","title":"Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"684b4eab1dc2c0057f679109770af0de9340af5cd933377b590298cf8a752956","revision_id":"4028d9c5-fb17-54c5-b72c-c6d21678a832","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}