{"id":"605a138f-e9b8-5f45-a553-8b14d302e6e1","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:clemastanin-b-influenza-subtype-range","predicate":"reduces","statement":"Clemastanin B inhibited human H1N1 including swine-origin H1N1, H3N2 and influenza B, and avian H6N2, H7N3 and H9N2, with inhibitory concentrations of 0.087 to 0.72 mg/mL, and was inactive against respiratory syncytial virus, adenovirus 3, parainfluenza virus 3, enterovirus 71 and human rhinovirus.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"d54add6f-d41a-590f-8637-542a5e7052df","mechanism_event_label":"Clemastanin B inhibited human H1N1 including swine-origin H1N1, H3N2 and influenza B, and avian H6N2, H7N3 and H9N2, with inhibitory concentrations of 0.087 to 0.72 mg/mL, and was inactive against respiratory syncytial virus, adenovirus 3, parainfluenza virus 3, enterovirus 71 and human rhinovirus.","subject":{"id":"0526af10-74ff-50d5-9602-125327302c48","slug":"clemastanin-b","display_name":"Clemastanin B","entity_type_key":"small_molecule"},"object":{"id":"17a1cd0d-3859-5c6b-a8d8-8db8cdd7151a","slug":"influenza-a-b-replication","display_name":"Influenza A and B virus replication in MDCK cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d54add6f-d41a-590f-8637-542a5e7052df","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:clemastanin-b-influenza-subtype-range-event","event_type":"observed_relationship","label":"Clemastanin B inhibited human H1N1 including swine-origin H1N1, H3N2 and influenza B, and avian H6N2, H7N3 and H9N2, with inhibitory concentrations of 0.087 to 0.72 mg/mL, and was inactive against respiratory syncytial virus, adenovirus 3, parainfluenza virus 3, enterovirus 71 and human rhinovirus.","description":"Clemastanin B inhibited human H1N1 including swine-origin H1N1, H3N2 and influenza B, and avian H6N2, H7N3 and H9N2, with inhibitory concentrations of 0.087 to 0.72 mg/mL, and was inactive against respiratory syncytial virus, adenovirus 3, parainfluenza virus 3, enterovirus 71 and human rhinovirus.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0526af10-74ff-50d5-9602-125327302c48","slug":"clemastanin-b","display_name":"Clemastanin B","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"17a1cd0d-3859-5c6b-a8d8-8db8cdd7151a","slug":"influenza-a-b-replication","display_name":"Influenza A and B virus replication in MDCK cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a5b9045b-d547-5e44-89c2-7440f928e90d","slug":"non-influenza-respiratory-virus-replication","display_name":"Replication of respiratory syncytial virus, adenovirus 3, parainfluenza 3, enterovirus 71 and human rhinovirus","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"MDCK cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Clemastanin B at 0.087 to 0.72 mg/mL","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The active range is in milligrams per millilitre, which is far above the concentrations at which the aglycone mechanisms operate. The inactivity against five unrelated viruses is recorded as a negative result in the same assay system.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"MDCK cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Clemastanin B inhibited human H1N1 including swine-origin H1N1, H3N2 and influenza B, and avian H6N2, H7N3 and H9N2, with inhibitory concentrations of 0.087 to 0.72 mg/mL, and was inactive against respiratory syncytial virus, adenovirus 3, parainfluenza virus 3, enterovirus 71 and human rhinovirus.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Antiviral activity of Isatis indigotica root-derived clemastanin B against human and avian influenza A and B viruses in vitro. (2013). https://pubmed.ncbi.nlm.nih.gov/23403777/ DOI: 10.3892/ijmm.2013.1274","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Virus replication across subtypes and unrelated respiratory viruses","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ec25d6cc-3a70-594c-a55f-0b7edce3c15a","evidence_kind":"source_excerpt","locator":"Lines 143-152","start_line":143,"end_line":152,"excerpt":"## clemastanin-b-influenza-subtype-range\nClemastanin B inhibited human H1N1 including swine-origin H1N1, H3N2 and influenza B, and avian H6N2, H7N3 and H9N2, with inhibitory concentrations of 0.087 to 0.72 mg/mL, and was inactive against respiratory syncytial virus, adenovirus 3, parainfluenza virus 3, enterovirus 71 and human rhinovirus.\nModel/species: MDCK cells\nTissue/system: Virus replication across subtypes and unrelated respiratory viruses\nExposure: Clemastanin B at 0.087 to 0.72 mg/mL\nRoute: In vitro\nDuration: Not stated here\nLimits: The active range is in milligrams per millilitre, which is far above the concentrations at which the aglycone mechanisms operate. The inactivity against five unrelated viruses is recorded as a negative result in the same assay system.\nPrimary reference: Antiviral activity of Isatis indigotica root-derived clemastanin B against human and avian influenza A and B viruses in vitro. (2013). https://pubmed.ncbi.nlm.nih.gov/23403777/ DOI: 10.3892/ijmm.2013.1274\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"48c58761-0291-5bad-a926-75e904b2d802","stable_key":"import-96d9034a-2550-5f3c-b767-1767c982533c","title":"Lariciresinol: five molecules under one name, and the mechanisms each one carries (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of twelve primary studies, every abstract read and all DOIs cross-checked against live PubMed metadata. Mechanism edges only, with no conclusion or claim of benefit recorded. Three author clusters account for eight of the twelve and carry shared laboratory keys. Study-specific concentrations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"6bf0015b7ff727b7a80d693dd18ffdd227d0e16ae45d9e85a5f81fa733edd3a4","revision_id":"219d9b0c-1a71-5e27-8d6c-7b3e77a594c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}