{"id":"5ff57e40-d834-5610-8851-4e75e49bdef1","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-thyroid-bioassay","predicate":"decreases_in_recorded_comparison","statement":"Preincubating bovine TSH with RA oxidation products reduced its activity in the mouse bioassay.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"1be9ceda-0f6c-5ec7-b082-9bd58d12eefa","mechanism_event_label":"Preincubating bovine TSH with RA oxidation products reduced its activity in the mouse bioassay.","subject":{"id":"7b72544b-d534-5919-b070-cd5edce404d8","slug":"ra-oxidation-products","display_name":"Rosmarinic-acid auto-oxidation products, mixture unresolved","entity_type_key":"chemical_species"},"object":{"id":"b434ae0e-2adc-5e53-af98-8cc185683640","slug":"mouse-tsh-bioactivity","display_name":"TSH bioactivity in the McKenzie mouse assay","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"1be9ceda-0f6c-5ec7-b082-9bd58d12eefa","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-thyroid-bioassay-event","event_type":"experimental_observation","label":"Preincubating bovine TSH with RA oxidation products reduced its activity in the mouse bioassay.","description":"### ra-thyroid-bioassay\n\nPreincubating bovine TSH with RA oxidation products reduced its activity in the mouse bioassay.\nThis does not establish hypothyroidism or interference with levothyroxine treatment.\nModel and exposure: Preincubation assay; not a controlled human oral RA trial.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: The active principles of plant extracts with antithyrotropic activity: oxidation products of derivatives of 3,4-dihydroxycinnamic acid. | 1985 | https://pubmed.ncbi.nlm.nih.gov/3987612/ | https://doi.org/10.1210/endo-116-5-1677 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7b72544b-d534-5919-b070-cd5edce404d8","slug":"ra-oxidation-products","display_name":"Rosmarinic-acid auto-oxidation products, mixture unresolved","entity_type_key":"chemical_species"},"role":"tested factor","stoichiometry":null,"state_label":"Hormone preincubated with oxidized RA","sequence_order":0,"notes":""},{"entity":{"id":"b434ae0e-2adc-5e53-af98-8cc185683640","slug":"mouse-tsh-bioactivity","display_name":"TSH bioactivity in the McKenzie mouse assay","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"role":"starting compound","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"eee708a0-a4ea-5489-b58c-e69029423bba","slug":"bovine-tsh","display_name":"Bovine thyrotropin","entity_type_key":"protein"},"role":"pretreated hormone","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Hormone preincubated with oxidized RA\", \"comparator\": \"Untreated hormone\", \"endpoint\": \"Preincubating bovine TSH with RA oxidation products reduced its activity in the mouse bioassay.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Preincubation assay; not a controlled human oral RA trial.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Rosmarinic acid collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Preincubating bovine TSH with RA oxidation products reduced its activity in the mouse bioassay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The active principles of plant extracts with antithyrotropic activity: oxidation products of derivatives of 3,4-dihydroxycinnamic acid. | https://pubmed.ncbi.nlm.nih.gov/3987612/ | https://doi.org/10.1210/endo-116-5-1677","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference ra-thyroid-bioassay; This does not establish hypothyroidism or interference with levothyroxine treatment.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"874d379c-def6-5ff9-91b3-0c0584508839","evidence_kind":"source_excerpt","locator":"Lines 510-516","start_line":510,"end_line":516,"excerpt":"### ra-thyroid-bioassay\n\nPreincubating bovine TSH with RA oxidation products reduced its activity in the mouse bioassay.\nThis does not establish hypothyroidism or interference with levothyroxine treatment.\nModel and exposure: Preincubation assay; not a controlled human oral RA trial.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: The active principles of plant extracts with antithyrotropic activity: oxidation products of derivatives of 3,4-dihydroxycinnamic acid. | 1985 | https://pubmed.ncbi.nlm.nih.gov/3987612/ | https://doi.org/10.1210/endo-116-5-1677 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"Preincubation assay; not a controlled human oral RA trial.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"606cff22-a6ed-59cd-a84d-87f44c59dc25","stable_key":"import-ca8e9e6a-6953-51ec-b712-2b06398a416d","title":"Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"684b4eab1dc2c0057f679109770af0de9340af5cd933377b590298cf8a752956","revision_id":"4028d9c5-fb17-54c5-b72c-c6d21678a832","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}