{"id":"5ff30bb4-e5b6-5afa-a472-092a1d095c23","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-e-cofilin-severing","predicate":"inhibits_tested","statement":"Cucurbitacin E pretreatment inhibited the F-actin-severing activity of purified human cofilin 1.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"5953e893-c6ba-5fdd-b062-df6656261342","mechanism_event_label":"Modifying the cutting protein slows filament disassembly.","subject":{"id":"d5631638-0ee3-537f-b7cc-cbd4958395d3","slug":"cucurbitacin-e","display_name":"Cucurbitacin E","entity_type_key":"small_molecule"},"object":{"id":"6509c007-6521-52c4-be1f-ce6bac8f156f","slug":"human-cofilin-actin-severing","display_name":"Human cofilin-mediated F-actin severing","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5953e893-c6ba-5fdd-b062-df6656261342","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-e-cofilin-severing-event","event_type":"observed_relationship","label":"Modifying the cutting protein slows filament disassembly.","description":"Cucurbitacin E pretreatment inhibited the F-actin-severing activity of purified human cofilin 1.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d5631638-0ee3-537f-b7cc-cbd4958395d3","slug":"cucurbitacin-e","display_name":"Cucurbitacin E","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6509c007-6521-52c4-be1f-ce6bac8f156f","slug":"human-cofilin-actin-severing","display_name":"Human cofilin-mediated F-actin severing","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b3d1d81d-9166-5e06-9e8e-c5136d5f874c","slug":"human-cfl1","display_name":"Human cofilin 1 / CFL1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"24579623-993e-5330-801b-b438cf595a0b","slug":"purified-cucurbitacin-f-actin","display_name":"Purified F-actin in cucurbitacin assays, species as reported","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text PMC3751690","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified-protein functional assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Actin preparation species is not resolved in this record; broad thiol reactivity prevents assigning cofilin as the exclusive cellular target.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Modifying the cutting protein slows filament disassembly.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cucurbitacin covalent bonding to cysteine thiols: the filamentous-actin severing protein Cofilin1 as an exemplary target. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23945128/ · DOI 10.1186/1478-811X-11-58","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"60e98b99-bd4b-55f4-8e2f-dc77949a75af","evidence_kind":"source_excerpt","locator":"Lines 44-50","start_line":44,"end_line":50,"excerpt":"## cucurbitacin-e-cofilin-severing\nModifying the cutting protein slows filament disassembly.\nCucurbitacin E pretreatment inhibited the F-actin-severing activity of purified human cofilin 1.\nModel: Purified-protein functional assay.\nLimitations: Actin preparation species is not resolved in this record; broad thiol reactivity prevents assigning cofilin as the exclusive cellular target.\nEvidence access: Primary full text PMC3751690\nCucurbitacin covalent bonding to cysteine thiols: the filamentous-actin severing protein Cofilin1 as an exemplary target. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23945128/ · DOI 10.1186/1478-811X-11-58","model_system":"Purified-protein functional assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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