{"id":"5fea92d9-fe33-5174-9149-4ddd8e5bf769","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-crat-null-assimilation","predicate":"knockout_did_not_reduce","statement":"Deleting CRAT did not diminish acetyl-CoA labeling from supplied acetylcarnitine in U87MG cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"f052c47d-06ba-56d8-b6f8-e9c86bffec7d","mechanism_event_label":"The enzyme needed to make acetylcarnitine need not be the one that uses it.","subject":{"id":"507b3d2c-db75-51d1-823a-0677e0c1a0e8","slug":"human-crat","display_name":"Human carnitine acetyltransferase / CRAT","entity_type_key":"protein"},"object":{"id":"cf898787-c987-53c5-a7c8-d5ae2cf75219","slug":"human-u87mg-acetyl-coa-labeling","display_name":"Human U87MG acetyl-CoA labeling from acetylcarnitine","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f052c47d-06ba-56d8-b6f8-e9c86bffec7d","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-crat-null-assimilation-event","event_type":"observed_relationship","label":"The enzyme needed to make acetylcarnitine need not be the one that uses it.","description":"Deleting CRAT did not diminish acetyl-CoA labeling from supplied acetylcarnitine in U87MG cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"507b3d2c-db75-51d1-823a-0677e0c1a0e8","slug":"human-crat","display_name":"Human carnitine acetyltransferase / CRAT","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cf898787-c987-53c5-a7c8-d5ae2cf75219","slug":"human-u87mg-acetyl-coa-labeling","display_name":"Human U87MG acetyl-CoA labeling from acetylcarnitine","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9ce82007-8418-514f-8b6f-9dd04c8b8b3d","slug":"human-crot","display_name":"Human carnitine octanoyltransferase / CROT","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full-text Figure 4","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human U87MG knockout and tracer experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Incoming acetylcarnitine utilization differs from mitochondrial production; not a conflict with muscle export.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Carnitine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The enzyme needed to make acetylcarnitine need not be the one that uses it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Carnitine octanoyltransferase is important for the assimilation of exogenous acetyl-L-carnitine into acetyl-CoA in mammalian cells. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36587768/ · DOI 10.1016/j.jbc.2022.102848","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"80959d11-9cc3-5fa0-8c04-96f2756b92f3","evidence_kind":"source_excerpt","locator":"Lines 226-232","start_line":226,"end_line":232,"excerpt":"## l-carnitine-crat-null-assimilation\nThe enzyme needed to make acetylcarnitine need not be the one that uses it.\nDeleting CRAT did not diminish acetyl-CoA labeling from supplied acetylcarnitine in U87MG cells.\nModel: Human U87MG knockout and tracer experiment.\nLimitations: Incoming acetylcarnitine utilization differs from mitochondrial production; not a conflict with muscle export.\nEvidence access: Primary full-text Figure 4\nCarnitine octanoyltransferase is important for the assimilation of exogenous acetyl-L-carnitine into acetyl-CoA in mammalian cells. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36587768/ · DOI 10.1016/j.jbc.2022.102848","model_system":"Human U87MG knockout and tracer experiment.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2c9c42b-806a-55e2-abde-b0320ea94704","stable_key":"import-6cdb37aa-8998-5ea8-829d-4f995caf98fc","title":"L-Carnitine: synthesis, acyl-group transport, fuel selection and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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