{"id":"5fad5e74-a5ea-5add-b61d-c133ef5c7331","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-pc-null-gluconeogenesis","predicate":"reduces","statement":"Liver-specific Pcx deletion reduced hepatic anaplerosis, TCA-cycle intermediates and gluconeogenesis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"37493ed4-726e-5883-ae3d-8a9d04900a0c","mechanism_event_label":"PC helps replenish the carbon pool needed for glucose production.","subject":{"id":"2a0aeed6-f0be-58b5-a300-93d994987939","slug":"mouse-liver-pc-null","display_name":"Liver-specific Pcx-null mouse genotype","entity_type_key":"gene"},"object":{"id":"cbd2bafa-f6d0-524f-a787-42c3fc4fff83","slug":"mouse-liver-gluconeogenesis","display_name":"Mouse hepatic gluconeogenesis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"37493ed4-726e-5883-ae3d-8a9d04900a0c","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-pc-null-gluconeogenesis-event","event_type":"biochemical_relationship","label":"PC helps replenish the carbon pool needed for glucose production.","description":"Liver-specific Pcx deletion reduced hepatic anaplerosis, TCA-cycle intermediates and gluconeogenesis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ac60d4e6-ce56-572e-9966-1676ea53c1db","slug":"mouse-pc","display_name":"Mouse pyruvate carboxylase / Pcx","entity_type_key":"protein"},"role":"deleted enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"enzyme cofactor context","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"32119d9f-54be-5e6d-98da-1621b0e2421d","slug":"oxaloacetate","display_name":"Oxaloacetate","entity_type_key":"small_molecule"},"role":"PC product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2a0aeed6-f0be-58b5-a300-93d994987939","slug":"mouse-liver-pc-null","display_name":"Liver-specific Pcx-null mouse genotype","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"cbd2bafa-f6d0-524f-a787-42c3fc4fff83","slug":"mouse-liver-gluconeogenesis","display_name":"Mouse hepatic gluconeogenesis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/biotin-research/31006591.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0dcedbfac623863bd4ae86ed1bb97cef0ef56bd417bd0d49507ef47d16af7b4e\", \"start_char\": 0, \"end_char\": 1141, \"text_sha256\": \"0dcedbfac623863bd4ae86ed1bb97cef0ef56bd417bd0d49507ef47d16af7b4e\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Liver-specific Pcx-knockout mice","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Genetic deletion of hepatic pyruvate carboxylase","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Genetic enzyme deletion is not a dietary biotin experiment. Cross-nutrient implications identify pathway dependence rather than a proven supplement response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Biotin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"PC helps replenish the carbon pool needed for glucose production.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b7-p31006591] Pyruvate-Carboxylase-Mediated Anaplerosis Promotes Antioxidant Capacity by Sustaining TCA Cycle and Redox Metabolism in Liver. (2019). https://pubmed.ncbi.nlm.nih.gov/31006591/ DOI: 10.1016/j.cmet.2019.03.014","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Liver and systemic metabolism","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"09db6427-8b73-5d06-8653-1c9184e08179","evidence_kind":"source_excerpt","locator":"Lines 819-830","start_line":819,"end_line":830,"excerpt":"### b7-pc-null-gluconeogenesis\nLiver-specific Pcx deletion reduced hepatic anaplerosis, TCA-cycle intermediates and gluconeogenesis.\nCondition category: machinery_impairment\nnutrient_topic: Biotin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: PC helps replenish the carbon pool needed for glucose production.\norganism: Mus musculus\ntissue_or_cell_type: Liver and systemic metabolism\nexperimental_model: Liver-specific Pcx-knockout mice\nlimitations: Genetic enzyme deletion is not a dietary biotin experiment. Cross-nutrient implications identify pathway dependence rather than a proven supplement response.\nexposure: Genetic deletion of hepatic pyruvate carboxylase\nevidence_span: {\"source_cache\": \"artifacts/biotin-research/31006591.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0dcedbfac623863bd4ae86ed1bb97cef0ef56bd417bd0d49507ef47d16af7b4e\", \"start_char\": 0, \"end_char\": 1141, \"text_sha256\": \"0dcedbfac623863bd4ae86ed1bb97cef0ef56bd417bd0d49507ef47d16af7b4e\"}\n[b7-p31006591] Pyruvate-Carboxylase-Mediated Anaplerosis Promotes Antioxidant Capacity by Sustaining TCA Cycle and Redox Metabolism in Liver. (2019). https://pubmed.ncbi.nlm.nih.gov/31006591/ DOI: 10.1016/j.cmet.2019.03.014","model_system":"Liver-specific Pcx-knockout mice","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b7-p31006591] Pyruvate-Carboxylase-Mediated Anaplerosis Promotes Antioxidant Capacity by Sustaining TCA Cycle and Redox Metabolism in Liver. (2019). https://pubmed.ncbi.nlm.nih.gov/31006591/ DOI: 10.1016/j.cmet.2019.03.014","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9608806b-adb6-5a35-b042-057147135642","stable_key":"import-08ce9896-9d1c-5bbf-b705-5bfe771091d5","title":"Biotin: carboxylases, recycling, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a05b23a45e0813ba2fcda0027e3f5d9d58b82858f8dd8598ffb7aca17e942a38","revision_id":"e0d0c2a2-e9e2-55dd-b467-41c22ba960d4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}