{"id":"5f8622c2-9f15-5a01-9d5f-af8441739443","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zn-sig-zinc-mtf1-binding","predicate":"enabled","statement":"Recombinant human and mouse MTF1 required added zinc for DNA binding; among the tested transition metals, only zinc activated recombinant MTF1 binding.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"37374918-feb2-5954-afc6-1f9ca8609a20","mechanism_event_label":"Zinc acted as a specific input to this metal-responsive transcription factor.","subject":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"object":{"id":"a2785dfa-f3a4-578e-ad13-7602cad6d828","slug":"mtf1-dna-binding","display_name":"MTF1 binding to metal-responsive DNA elements","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"37374918-feb2-5954-afc6-1f9ca8609a20","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zn-sig-zinc-mtf1-binding-event","event_type":"biochemical_relationship","label":"Zinc acted as a specific input to this metal-responsive transcription factor.","description":"Recombinant human and mouse MTF1 required added zinc for DNA binding; among the tested transition metals, only zinc activated recombinant MTF1 binding.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1a6ae94b-21bd-5800-8eba-de39db9eeac9","slug":"mtf1","display_name":"Human metal-regulatory transcription factor 1 / MTF1","entity_type_key":"protein"},"role":"human_protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e83dc538-b8b1-5528-9807-277f5b646893","slug":"mouse-mtf1","display_name":"Mouse metal-regulatory transcription factor 1 / Mtf1","entity_type_key":"protein"},"role":"mouse_protein","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a2785dfa-f3a4-578e-ad13-7602cad6d828","slug":"mtf1-dna-binding","display_name":"MTF1 binding to metal-responsive DNA elements","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Human metal-regulatory transcription factor 1 / MTF1 (human_protein); Mouse metal-regulatory transcription factor 1 / Mtf1 (mouse_protein)","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/zinc-signaling-sources/9507026-abstract.txt\", \"locator\": \"Primary indexed abstract\", \"file_sha256\": \"ecd19e16b15909d46328c2c5dcd9f9536422db7bb12b9dfd9d91763fafe164bd\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human and mouse cell extracts and recombinant MTF1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Zinc versus other tested transition metals; DNA-binding assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"In-vitro metal specificity does not mean all cellular metal responses are mediated by direct MTF1 binding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zinc research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens; Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Zinc acted as a specific input to this metal-responsive transcription factor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zn-sig-9507026] The DNA binding activity of metal response element-binding transcription factor-1 is activated in vivo and in vitro by zinc, but not by other transition metals. (1998). https://pubmed.ncbi.nlm.nih.gov/9507026/ DOI: 10.1074/jbc.273.12.7127","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell extracts and recombinant protein","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8ed2db69-b90b-519b-b644-8ea6b681bbfb","evidence_kind":"source_excerpt","locator":"Lines 873-885","start_line":873,"end_line":885,"excerpt":"### zn-sig-zinc-mtf1-binding\nRecombinant human and mouse MTF1 required added zinc for DNA binding; among the tested transition metals, only zinc activated recombinant MTF1 binding.\nCondition category: normal\nnutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Zinc acted as a specific input to this metal-responsive transcription factor.\norganism: Homo sapiens; Mus musculus\ntissue_or_cell_type: Cell extracts and recombinant protein\nexperimental_model: Human and mouse cell extracts and recombinant MTF1\nlimitations: In-vitro metal specificity does not mean all cellular metal responses are mediated by direct MTF1 binding.\nexposure: Zinc versus other tested transition metals; DNA-binding assays.\ncross_nutrient: Human metal-regulatory transcription factor 1 / MTF1 (human_protein); Mouse metal-regulatory transcription factor 1 / Mtf1 (mouse_protein)\nevidence_span: {\"source_cache\": \"artifacts/zinc-signaling-sources/9507026-abstract.txt\", \"locator\": \"Primary indexed abstract\", \"file_sha256\": \"ecd19e16b15909d46328c2c5dcd9f9536422db7bb12b9dfd9d91763fafe164bd\"}\n[zn-sig-9507026] The DNA binding activity of metal response element-binding transcription factor-1 is activated in vivo and in vitro by zinc, but not by other transition metals. (1998). https://pubmed.ncbi.nlm.nih.gov/9507026/ DOI: 10.1074/jbc.273.12.7127","model_system":"Human and mouse cell extracts and recombinant MTF1","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zn-sig-9507026] The DNA binding activity of metal response element-binding transcription factor-1 is activated in vivo and in vitro by zinc, but not by other transition metals. (1998). https://pubmed.ncbi.nlm.nih.gov/9507026/ DOI: 10.1074/jbc.273.12.7127","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c5ee0fee-ce5c-58de-905a-10fb0ea0723c","stable_key":"import-6d38d43e-01e4-5641-93be-65654271e242","title":"Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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