{"id":"5f6b20a7-8af5-558f-8a99-b87a9d6e6ba1","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-trans-zip14-copper-negative","predicate":"does_not_increase","statement":"Mouse ZIP14 did not increase uptake of radiolabeled copper supplied as Cu(I) or Cu(II) in the tested oocyte conditions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"81007242-8d8a-503d-950c-c2f4dcf978ce","mechanism_event_label":"This ZIP14 experiment found no copper transport despite detecting other metal substrates.","subject":{"id":"9e0e70ec-aa5b-584d-a6ea-28c400cb3100","slug":"mouse-slc39a14","display_name":"Mouse ZIP14 (Slc39a14)","entity_type_key":"protein"},"object":{"id":"00ad835a-b52b-5ea9-ba2c-db4b73c00cdd","slug":"cellular-copper-uptake","display_name":"Cellular copper uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"81007242-8d8a-503d-950c-c2f4dcf978ce","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-trans-zip14-copper-negative-event","event_type":"biochemical_relationship","label":"This ZIP14 experiment found no copper transport despite detecting other metal substrates.","description":"Mouse ZIP14 did not increase uptake of radiolabeled copper supplied as Cu(I) or Cu(II) in the tested oocyte conditions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"162020b5-1fa5-526e-8405-452891245722","slug":"copper-i","display_name":"Copper(I) ion","entity_type_key":"ion"},"role":"tested_nontransported_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2a870bb5-05a0-5e51-a70c-a9a843a8b571","slug":"copper-ii","display_name":"Copper(II) ion","entity_type_key":"ion"},"role":"tested_nontransported_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"positive_substrate_context","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9e0e70ec-aa5b-584d-a6ea-28c400cb3100","slug":"mouse-slc39a14","display_name":"Mouse ZIP14 (Slc39a14)","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"00ad835a-b52b-5ea9-ba2c-db4b73c00cdd","slug":"cellular-copper-uptake","display_name":"Cellular copper uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Radiolabeled Cu(I) and Cu(II) uptake in Xenopus oocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Cu(I) and Cu(II) tested separately against controls.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A bounded negative result does not mean zinc and copper have no interaction through other proteins or intestinal mechanisms.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zinc research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse protein in Xenopus laevis oocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This ZIP14 experiment found no copper transport despite detecting other metal substrates.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zinc-trans-21653899] Zip14 is a complex broad-scope metal-ion transporter whose functional properties support roles in the cellular uptake of zinc and nontransferrin-bound iron. (2011). https://pubmed.ncbi.nlm.nih.gov/21653899/ DOI: 10.1152/ajpcell.00479.2010","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Oocyte plasma membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"20179e85-48a7-514e-8b40-76e885685aa9","evidence_kind":"source_excerpt","locator":"Lines 518-529","start_line":518,"end_line":529,"excerpt":"### zinc-trans-zip14-copper-negative\nMouse ZIP14 did not increase uptake of radiolabeled copper supplied as Cu(I) or Cu(II) in the tested oocyte conditions.\nCondition category: normal\nnutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This ZIP14 experiment found no copper transport despite detecting other metal substrates.\norganism: Mouse protein in Xenopus laevis oocytes\ntissue_or_cell_type: Oocyte plasma membrane\nexperimental_model: Radiolabeled Cu(I) and Cu(II) uptake in Xenopus oocytes\nlimitations: A bounded negative result does not mean zinc and copper have no interaction through other proteins or intestinal mechanisms.\nexposure: Cu(I) and Cu(II) tested separately against controls.\ncross_nutrient: true\n[zinc-trans-21653899] Zip14 is a complex broad-scope metal-ion transporter whose functional properties support roles in the cellular uptake of zinc and nontransferrin-bound iron. (2011). https://pubmed.ncbi.nlm.nih.gov/21653899/ DOI: 10.1152/ajpcell.00479.2010","model_system":"Radiolabeled Cu(I) and Cu(II) uptake in Xenopus oocytes","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zinc-trans-21653899] Zip14 is a complex broad-scope metal-ion transporter whose functional properties support roles in the cellular uptake of zinc and nontransferrin-bound iron. (2011). https://pubmed.ncbi.nlm.nih.gov/21653899/ DOI: 10.1152/ajpcell.00479.2010","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c5ee0fee-ce5c-58de-905a-10fb0ea0723c","stable_key":"import-6d38d43e-01e4-5641-93be-65654271e242","title":"Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"2e731dd54477ec1254e97df3323e1208d38f1375effed19252e71ab4f600d13a","revision_id":"c72258b9-ac09-5408-9d44-a921ad1f96a3","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}