{"id":"5f2d88d1-a228-595f-8550-bbd616215892","stable_key":"4910e17b-aaec-52cd-b9e5-caca85d817ee:red-yeast-rice-ankaflavin-nrf2","predicate":"increases_tested_phosphorylation_of","statement":"Ankaflavin increased hepatic Nrf2 Ser40 phosphorylation without increasing measured Nrf2 mRNA or total protein in the rat experiment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7cb1d82a-a3fa-5c41-a8a4-895a7d604425","mechanism_event_label":"Activation and abundance were different measured endpoints.","subject":{"id":"92939faa-84d1-52e8-a70f-deefd2486d52","slug":"ankaflavin","display_name":"Ankaflavin","entity_type_key":"small_molecule"},"object":{"id":"27636f1d-fd51-5697-a8bb-6c827f4f8b40","slug":"rat-nfe2l2","display_name":"Rat Nrf2 / Nfe2l2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"7cb1d82a-a3fa-5c41-a8a4-895a7d604425","stable_key":"4910e17b-aaec-52cd-b9e5-caca85d817ee:red-yeast-rice-ankaflavin-nrf2-event","event_type":"observed_relationship","label":"Activation and abundance were different measured endpoints.","description":"Ankaflavin increased hepatic Nrf2 Ser40 phosphorylation without increasing measured Nrf2 mRNA or total protein in the rat experiment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"92939faa-84d1-52e8-a70f-deefd2486d52","slug":"ankaflavin","display_name":"Ankaflavin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"27636f1d-fd51-5697-a8bb-6c827f4f8b40","slug":"rat-nfe2l2","display_name":"Rat Nrf2 / Nfe2l2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Methylglyoxal-treated Wistar rats, four-week model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not demonstrate direct binding to Nrf2 or KEAP1; effect cannot be assigned to dietary riboflavin.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Red yeast rice collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"red-yeast-rice","display_name":"Red yeast rice","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Activation and abundance were different measured endpoints.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[23022408] Ankaflavin: a natural novel PPARγ agonist upregulates Nrf2 to attenuate methylglyoxal-induced diabetes in vivo. · 2012 · https://pubmed.ncbi.nlm.nih.gov/23022408/ · DOI 10.1016/j.freeradbiomed.2012.09.025","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"574c7f5c-2383-50c3-ad57-df08277d53a3","evidence_kind":"source_excerpt","locator":"Lines 244-250","start_line":244,"end_line":250,"excerpt":"## red-yeast-rice-ankaflavin-nrf2\nActivation and abundance were different measured endpoints.\nAnkaflavin increased hepatic Nrf2 Ser40 phosphorylation without increasing measured Nrf2 mRNA or total protein in the rat experiment.\nModel: Methylglyoxal-treated Wistar rats, four-week model.\nLimitations: Does not demonstrate direct binding to Nrf2 or KEAP1; effect cannot be assigned to dietary riboflavin.\nEvidence access: Primary abstract\n[23022408] Ankaflavin: a natural novel PPARγ agonist upregulates Nrf2 to attenuate methylglyoxal-induced diabetes in vivo. · 2012 · https://pubmed.ncbi.nlm.nih.gov/23022408/ · DOI 10.1016/j.freeradbiomed.2012.09.025","model_system":"Methylglyoxal-treated Wistar rats, four-week model.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"261a12bc-3d00-52a4-a5ec-70e174390596","stable_key":"import-4910e17b-aaec-52cd-b9e5-caca85d817ee","title":"Red yeast rice: constituents, mevalonate, CoQ and product-specific interactions (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"271bbcecaaacc76ad4d06718ae592dc6a1b33e3e459adfdd36cbd0ac5f09c0f6","revision_id":"9c30956c-206c-5c49-ae42-81b070088e26","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}