{"id":"5ef0c131-0946-53f5-8a96-904baaf56bcc","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-act-tocopherol-uptake-competition","predicate":"reduces_uptake","statement":"Co-incubation with tocopherol significantly impaired cholecalciferol uptake in Caco-2 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"1269aea7-0001-5788-b677-249333221f2e","mechanism_event_label":"Vitamin E competed with D3 uptake in an intestinal cell model.","subject":{"id":"40a6525a-76b8-576e-b73d-1e11aecf5f8d","slug":"alpha-tocopherol","display_name":"Alpha-tocopherol","entity_type_key":"small_molecule"},"object":{"id":"07f07b97-3fa1-5633-95b9-8f4cc0c11a23","slug":"intestinal-cholecalciferol-uptake","display_name":"Intestinal cholecalciferol uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"1269aea7-0001-5788-b677-249333221f2e","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-act-tocopherol-uptake-competition-event","event_type":"biochemical_relationship","label":"Vitamin E competed with D3 uptake in an intestinal cell model.","description":"Co-incubation with tocopherol significantly impaired cholecalciferol uptake in Caco-2 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"40a6525a-76b8-576e-b73d-1e11aecf5f8d","slug":"alpha-tocopherol","display_name":"Alpha-tocopherol","entity_type_key":"small_molecule"},"role":"competing_nutrient","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b162e431-b442-5400-9335-ec91f7875a4e","slug":"cholecalciferol","display_name":"Cholecalciferol","entity_type_key":"small_molecule"},"role":"uptake_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"07f07b97-3fa1-5633-95b9-8f4cc0c11a23","slug":"intestinal-cholecalciferol-uptake","display_name":"Intestinal cholecalciferol uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Primary abstract, Methods and results; exact incubation concentrations/durations unavailable in abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Caco-2 apical uptake assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Tocopherol and cholecalciferol co-incubation; dose/time unavailable in primary abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The primary abstract says tocopherol; the authors' subsequent primary paper identifies it as alpha-tocopherol. No evidence here warrants separating normal oral supplements or diagnosing D malabsorption.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient","value_text":"Vitamin D2 and D3","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"nutrient_topic","value_text":"Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Vitamin E competed with D3 uptake in an intestinal cell model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[reboul2011] Vitamin D intestinal absorption is not a simple passive diffusion: evidences for involvement of cholesterol transporters. (2011). https://pubmed.ncbi.nlm.nih.gov/21280209/ DOI: 10.1002/mnfr.201000553\n[goncalves2015] Fat-soluble vitamin intestinal absorption: absorption sites in the intestine and interactions for absorption. (2015). https://pubmed.ncbi.nlm.nih.gov/25442537/ DOI: 10.1016/j.foodchem.2014.09.021","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Caco-2 intestinal epithelial model","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8f56e315-e71e-56c4-8e9a-c12b379bd6bd","evidence_kind":"source_excerpt","locator":"Lines 211-225","start_line":211,"end_line":225,"excerpt":"### vd-act-tocopherol-uptake-competition\nCo-incubation with tocopherol significantly impaired cholecalciferol uptake in Caco-2 cells.\nCondition category: normal\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Vitamin E competed with D3 uptake in an intestinal cell model.\norganism: Homo sapiens\ntissue_or_cell_type: Caco-2 intestinal epithelial model\nexperimental_model: Caco-2 apical uptake assay\nlimitations: The primary abstract says tocopherol; the authors' subsequent primary paper identifies it as alpha-tocopherol. No evidence here warrants separating normal oral supplements or diagnosing D malabsorption.\nexposure: Tocopherol and cholecalciferol co-incubation; dose/time unavailable in primary abstract.\ncross_nutrient: true\nevidence_location: Primary abstract, Methods and results; exact incubation concentrations/durations unavailable in abstract.\nnutrient: Vitamin D2 and D3\n[reboul2011] Vitamin D intestinal absorption is not a simple passive diffusion: evidences for involvement of cholesterol transporters. (2011). https://pubmed.ncbi.nlm.nih.gov/21280209/ DOI: 10.1002/mnfr.201000553\n[goncalves2015] Fat-soluble vitamin intestinal absorption: absorption sites in the intestine and interactions for absorption. (2015). https://pubmed.ncbi.nlm.nih.gov/25442537/ DOI: 10.1016/j.foodchem.2014.09.021","model_system":"Caco-2 apical uptake assay","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [reboul2011] Vitamin D intestinal absorption is not a simple passive diffusion: evidences for involvement of cholesterol transporters. (2011). https://pubmed.ncbi.nlm.nih.gov/21280209/ DOI: 10.1002/mnfr.201000553; [goncalves2015] Fat-soluble vitamin intestinal absorption: absorption sites in the intestine and interactions for absorption. (2015). https://pubmed.ncbi.nlm.nih.gov/25442537/ DOI: 10.1016/j.foodchem.2014.09.021","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c95c763c-5b31-5b3f-a2f8-2dfe5ef0d3ca","stable_key":"import-1deb434a-3547-51a0-a038-87b1ce79ec38","title":"Vitamin D2 and D3: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"51004bf0d78db6146b8f198b0688a3cc7d4ea9822415a9c034f4c8790fb4e38b","revision_id":"d52ff207-a311-5f24-96f4-462838792db5","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}