{"id":"5e7dbc08-50c7-5834-9e13-b37406990133","stable_key":"3ad23a58-d6a8-5554-81d6-9c83abd33087:znt8-granules","predicate":"reported_comparison","statement":"SLC30A8-null and R138X beta cells had fewer crystalline insulin granules; glucose-stimulated secretion was not impaired in the tested system.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b8a633f7-2c63-54ba-a3be-a6f2627f9059","mechanism_event_label":"SLC30A8-null and R138X beta cells had fewer crystalline insulin granules; glucose-stimulated secretion was not impaired in the tested system.","subject":{"id":"5ec5ce4e-ee24-5bff-a525-5e57fc4db7c3","slug":"human-slc30a8-null-beta-cells","display_name":"Human stem-cell-derived SLC30A8-null beta cells","entity_type_key":"cell_type"},"object":{"id":"ec245b55-7b6e-5287-9376-a6dd05d257b0","slug":"human-beta-cell-insulin-granule-crystallization","display_name":"Human beta-cell insulin-granule crystallization","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b8a633f7-2c63-54ba-a3be-a6f2627f9059","stable_key":"3ad23a58-d6a8-5554-81d6-9c83abd33087:znt8-granules","event_type":"reported_comparison","label":"SLC30A8-null and R138X beta cells had fewer crystalline insulin granules; glucose-stimulated secretion was not impaired in the tested system.","description":"","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5ec5ce4e-ee24-5bff-a525-5e57fc4db7c3","slug":"human-slc30a8-null-beta-cells","display_name":"Human stem-cell-derived SLC30A8-null beta cells","entity_type_key":"cell_type"},"role":"experimental_actor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ec245b55-7b6e-5287-9376-a6dd05d257b0","slug":"human-beta-cell-insulin-granule-crystallization","display_name":"Human beta-cell insulin-granule crystallization","entity_type_key":"cellular_process"},"role":"measured_endpoint","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a4da847b-7430-527e-bc73-8d17fd5255ba","slug":"slc30a8","display_name":"Human zinc transporter ZnT8 / SLC30A8","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7c50efe8-650e-5145-81ef-1c430510a538","slug":"insulin-secretion","display_name":"Insulin secretion","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e0dc1af6-0068-5ae2-8ab0-a5199252ce7d","slug":"human-slc30a8-r138x-beta-cells","display_name":"Human stem-cell-derived SLC30A8 R138X beta cells","entity_type_key":"cell_type"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"curation_topic","value_text":"zinc","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"experimental_condition","value_text":"SLC30A8-null or R138X cells","comparator":"Wild-type cells","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"human-slc30a8-null-beta-cells","display_name":"Human stem-cell-derived SLC30A8-null beta cells","entity_type_key":"cell_type"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"SLC30A8-null or R138X cells\", \"comparator\": \"Wild-type cells\", \"endpoint\": \"Granule crystallization\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": [{\"entity_slug\": \"human-slc30a8-null-beta-cells\", \"state\": \"SLC30A8-null or R138X cells\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Engineered human embryonic stem-cell-derived beta cells; results and figure 5 reviewed","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Separate genotypes, not a combined knockout. Preserved secretion does not establish universal protection.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"SLC30A8 stem-cell study; 2023; PMCID:PMC10047077; https://pmc.ncbi.nlm.nih.gov/articles/PMC10047077/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"8aad5a42-14b1-5cda-80ed-9bca59445c55","evidence_kind":"source_excerpt","locator":"Lines 12-12","start_line":12,"end_line":12,"excerpt":"SLC30A8-null and R138X beta cells had fewer crystalline insulin granules; glucose-stimulated secretion was not impaired in the tested system. Model: Engineered human embryonic stem-cell-derived beta cells; results and figure 5 reviewed. Limits: Separate genotypes, not a combined knockout. Preserved secretion does not establish universal protection. 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