{"id":"5e288224-51b3-5a5e-97dc-3d84d79c9da2","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:ca-ryr1-calcium-gating","predicate":"regulates","statement":"Calcium binds an activating RyR1 site and primes gating; ATP and caffeine increase open-state representation in the experimental preparation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fdc9edfd-6264-5ce6-86ad-636a07539f26","mechanism_event_label":"Calcium helps regulate the RyR1 gate.","subject":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"object":{"id":"e69a2b9f-66f0-53a8-b6a2-d10256e17f91","slug":"ryr1","display_name":"Ryanodine receptor 1 / RYR1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"fdc9edfd-6264-5ce6-86ad-636a07539f26","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:ca-ryr1-calcium-gating-event","event_type":"regulation","label":"Calcium helps regulate the RyR1 gate.","description":"Calcium binds an activating RyR1 site and primes gating; 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presence here is not a separate transport or causal claim."}]},"contexts":[{"dimension":"compartment_description","value_text":"Cytosolic channel-regulatory region","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified rabbit skeletal-muscle RyR1 with calstabin2; cryo-EM and planar-bilayer recordings","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Calcium is both a transported ion and a ligand; caffeine-assisted structures do not imply caffeine is required physiologically.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Calcium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Oryctolagus cuniculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Calcium helps regulate the RyR1 gate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ca-desgeorges2016] Structural Basis for Gating and Activation of RyR1 (2016). https://pubmed.ncbi.nlm.nih.gov/27662087/ DOI: 10.1016/j.cell.2016.08.075","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"research_relationship_category","value_text":"regulation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Skeletal-muscle channel preparation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1d6f952c-a8fc-56fa-9583-060662fcf5ae","evidence_kind":"source_excerpt","locator":"Lines 666-677","start_line":666,"end_line":677,"excerpt":"### ca-ryr1-calcium-gating\nCalcium binds an activating RyR1 site and primes gating; ATP and caffeine increase open-state representation in the experimental preparation.\nCondition category: normal\nnutrient_topic: Calcium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Calcium helps regulate the RyR1 gate.\norganism: Oryctolagus cuniculus\ntissue_or_cell_type: Skeletal-muscle channel preparation\nexperimental_model: Purified rabbit skeletal-muscle RyR1 with calstabin2; cryo-EM and planar-bilayer recordings\nlimitations: Calcium is both a transported ion and a ligand; caffeine-assisted structures do not imply caffeine is required physiologically.\nresearch_relationship_category: regulation\ncompartment_description: Cytosolic channel-regulatory region\n[ca-desgeorges2016] Structural Basis for Gating and Activation of RyR1 (2016). https://pubmed.ncbi.nlm.nih.gov/27662087/ DOI: 10.1016/j.cell.2016.08.075","model_system":"Purified rabbit skeletal-muscle RyR1 with calstabin2; cryo-EM and planar-bilayer recordings","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ca-desgeorges2016] Structural Basis for Gating and Activation of RyR1 (2016). https://pubmed.ncbi.nlm.nih.gov/27662087/ DOI: 10.1016/j.cell.2016.08.075","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3c083fa0-59c6-50e9-af42-678d0e3c1006","stable_key":"import-434e1e27-eb90-583a-956b-62472726ffba","title":"Calcium: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"965c934d6b147f2b62f8d45ce6d7a87681a600bfe5c46a585b4704889afd3fc1","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}