{"id":"5e20035f-bda9-58ca-b12d-0152c2d16e07","stable_key":"475ea55a-65e9-51c3-a539-738a6a8f683f:mangiferin-acute-pdh","predicate":"decreases","statement":"After 15 minutes, PDH phosphorylation decreased without a detected change in PDK4 abundance.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c33642a8-415e-5744-a628-abf9978fb050","mechanism_event_label":"The rapid response differed from the later change in protein abundance.","subject":{"id":"2cfc0bd9-b5cc-58ab-852a-7705f3d8d47f","slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"},"object":{"id":"aaa557b1-ebb3-55ea-9284-e88dc55c5245","slug":"mouse-pdh-phosphorylation","display_name":"Mouse muscle pyruvate dehydrogenase phosphorylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c33642a8-415e-5744-a628-abf9978fb050","stable_key":"475ea55a-65e9-51c3-a539-738a6a8f683f:mangiferin-acute-pdh-event","event_type":"biochemical_relationship","label":"The rapid response differed from the later change in protein abundance.","description":"After 15 minutes, PDH phosphorylation decreased without a detected change in PDK4 abundance.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2cfc0bd9-b5cc-58ab-852a-7705f3d8d47f","slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"aaa557b1-ebb3-55ea-9284-e88dc55c5245","slug":"mouse-pdh-phosphorylation","display_name":"Mouse muscle pyruvate dehydrogenase phosphorylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/mangiferin-research/24848064.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"8cbbc4a9b075ae11f5b88a9929a65c77e6b39870068e320a281bd1802384fec8\", \"start_char\": 30977, \"end_char\": 31862, \"text_sha256\": \"8e9b4448f5213862fa4c8b78f1d49c9f6b4e6faffb97d818dabbd4117d386dba\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"High-fat-fed mice and differentiated C2C12 myotube experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"200 or 1000 micromolar mangiferin, 15 minutes; Figure 6","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The authors proposed inhibition of kinase activity; this observation alone does not identify a direct molecular target.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Mangiferin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The rapid response differed from the later change in protein abundance.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mangiferin-p24848064] Mangiferin stimulates carbohydrate oxidation and protects against metabolic disorders induced by high-fat diets. (2014). https://pubmed.ncbi.nlm.nih.gov/24848064/ DOI: 10.2337/db14-0006","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Skeletal muscle and cultured myotubes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f4941963-3127-5831-89b3-b62d3b069429","evidence_kind":"source_excerpt","locator":"Lines 366-377","start_line":366,"end_line":377,"excerpt":"### mangiferin-acute-pdh\nAfter 15 minutes, PDH phosphorylation decreased without a detected change in PDK4 abundance.\nCondition category: normal\nnutrient_topic: Mangiferin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The rapid response differed from the later change in protein abundance.\norganism: Mus musculus\ntissue_or_cell_type: Skeletal muscle and cultured myotubes\nexperimental_model: High-fat-fed mice and differentiated C2C12 myotube experiments\nlimitations: The authors proposed inhibition of kinase activity; this observation alone does not identify a direct molecular target.\nexposure: 200 or 1000 micromolar mangiferin, 15 minutes; Figure 6\nevidence_span: {\"source_cache\": \"artifacts/mangiferin-research/24848064.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"8cbbc4a9b075ae11f5b88a9929a65c77e6b39870068e320a281bd1802384fec8\", \"start_char\": 30977, \"end_char\": 31862, \"text_sha256\": \"8e9b4448f5213862fa4c8b78f1d49c9f6b4e6faffb97d818dabbd4117d386dba\"}\n[mangiferin-p24848064] Mangiferin stimulates carbohydrate oxidation and protects against metabolic disorders induced by high-fat diets. (2014). https://pubmed.ncbi.nlm.nih.gov/24848064/ DOI: 10.2337/db14-0006","model_system":"High-fat-fed mice and differentiated C2C12 myotube experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mangiferin-p24848064] Mangiferin stimulates carbohydrate oxidation and protects against metabolic disorders induced by high-fat diets. (2014). https://pubmed.ncbi.nlm.nih.gov/24848064/ DOI: 10.2337/db14-0006","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"267f942a-695a-5b62-a858-f71c06321ff5","stable_key":"import-475ea55a-65e9-51c3-a539-738a6a8f683f","title":"Mangiferin: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"295cb3a13742b3caeed19347ffcbc7863f5372d983da1625a942428919b4dc98","revision_id":"d9c2ee89-ff22-5e86-9d7f-7cb7e17f9ed2","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}