{"id":"5e008bed-8650-58c5-92a3-9243acc9c5a7","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:yeast-metal-loss-yeast-ctr1","predicate":"decreases_in_recorded_experiment","statement":"Deletion of yeast-ctr1 increased yeast sensitivity to allicin.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"a4715cb9-2108-536b-a303-50eb0d4ff27d","mechanism_event_label":"Deletion of yeast-ctr1 increased yeast sensitivity to allicin.","subject":{"id":"573821cf-edbe-5fa1-8a7c-ed2cc9e713a7","slug":"yeast-ctr1","display_name":"Saccharomyces cerevisiae high-affinity copper transporter / Ctr1","entity_type_key":"protein"},"object":{"id":"29ccc26a-b3de-57b2-b46d-5f6206054867","slug":"yeast-allicin-growth","display_name":"Saccharomyces cerevisiae growth during allicin exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a4715cb9-2108-536b-a303-50eb0d4ff27d","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:yeast-metal-loss-yeast-ctr1-event","event_type":"experimental_observation","label":"Deletion of yeast-ctr1 increased yeast sensitivity to allicin.","description":"**Yeast metal requirements.** In a chemical-genetic screen of Saccharomyces cerevisiae, loss of CTR1, MAC1, or ZAP1 increased sensitivity to allicin; copper or zinc additions rescued the relevant sensitized phenotypes. This supports involvement of metal acquisition or metalloprotein function but does not discriminate those alternatives. It is not direct proof that allicin binds each protein, chelates all intracellular copper, or causes human mineral deficiency. Exact supplementation concentrations and all strain-specific contrasts remain unextracted from the accessible abstract. [Prescott and Panaretou 2017](https://pubmed.ncbi.nlm.nih.gov/28421744/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"573821cf-edbe-5fa1-8a7c-ed2cc9e713a7","slug":"yeast-ctr1","display_name":"Saccharomyces cerevisiae high-affinity copper transporter / Ctr1","entity_type_key":"protein"},"role":"tested factor","stoichiometry":null,"state_label":"yeast-ctr1 deletion with allicin","sequence_order":0,"notes":""},{"entity":{"id":"29ccc26a-b3de-57b2-b46d-5f6206054867","slug":"yeast-allicin-growth","display_name":"Saccharomyces cerevisiae growth during allicin exposure","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"role":"test compound","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary indexed abstract reviewed; full methods, exact concentrations or species-specific attribution remain unextracted unless explicitly stated in the abstract or separately verified publisher text.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"present","comparator":"Yeast comparator without that deletion at matched allicin exposure","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"deleted","comparator":"Yeast comparator without that deletion at matched allicin exposure","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"yeast-ctr1","display_name":"Saccharomyces cerevisiae high-affinity copper transporter / Ctr1","entity_type_key":"protein"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"yeast-ctr1 deletion with allicin\", \"comparator\": \"Yeast comparator without that deletion at matched allicin exposure\", \"endpoint\": \"Deletion of yeast-ctr1 increased yeast sensitivity to allicin.\", \"effect_direction\": \"decrease\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"yeast-ctr1\", \"state\": \"deleted\"}, {\"entity_slug\": \"allicin\", \"state\": \"present\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Saccharomyces cerevisiae deletion screen; primary abstract; exact dose and exposure duration unextracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Hypersensitivity does not prove direct binding to the deleted protein or distinguish metal uptake from metalloprotein dysfunction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Deletion of yeast-ctr1 increased yeast sensitivity to allicin.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A Mini HIP HOP Assay Uncovers a Central Role for Copper and Zinc in the Antifungal Mode of Action of Allicin. | 2017 | DOI 10.1021/acs.jafc.7b00250 | PMID 28421744 | https://pubmed.ncbi.nlm.nih.gov/28421744/ | https://doi.org/10.1021/acs.jafc.7b00250","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 59-59; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"f3815b8a-5223-5410-8c39-62309b9cbc93","evidence_kind":"source_excerpt","locator":"Lines 59-59","start_line":59,"end_line":59,"excerpt":"**Yeast metal requirements.** In a chemical-genetic screen of Saccharomyces cerevisiae, loss of CTR1, MAC1, or ZAP1 increased sensitivity to allicin; copper or zinc additions rescued the relevant sensitized phenotypes. This supports involvement of metal acquisition or metalloprotein function but does not discriminate those alternatives. It is not direct proof that allicin binds each protein, chelates all intracellular copper, or causes human mineral deficiency. Exact supplementation concentrations and all strain-specific contrasts remain unextracted from the accessible abstract. [Prescott and Panaretou 2017](https://pubmed.ncbi.nlm.nih.gov/28421744/)","model_system":"Saccharomyces cerevisiae deletion screen; primary abstract; exact dose and exposure duration unextracted.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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