{"id":"5de3e34c-4659-5834-9df9-6dd482ba6c92","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-mouse-intestinal-slc1a3","predicate":"increases_intestinal_expression_of","statement":"The study reported increased mRNA and protein expression of the mouse intestinal transporter component SLC1A3.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"28817411-3c32-50f7-84bc-40a31baf4f44","mechanism_event_label":"Each transporter remains searchable separately from the overall amino acid response.","subject":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"object":{"id":"f70186a4-d3e6-55ea-877d-df76b0f18ce7","slug":"mouse-slc1a3","display_name":"Mouse EAAT1 / Slc1a3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"28817411-3c32-50f7-84bc-40a31baf4f44","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-mouse-intestinal-slc1a3-event","event_type":"biochemical_relationship","label":"Each transporter remains searchable separately from the overall amino acid response.","description":"The study reported increased mRNA and protein expression of the mouse intestinal transporter component SLC1A3.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f70186a4-d3e6-55ea-877d-df76b0f18ce7","slug":"mouse-slc1a3","display_name":"Mouse EAAT1 / Slc1a3","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/theanine-research/36615799.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0bb37e6a5f3624dc13eb87705f21721a6cf7cc2151b0ecf87dedef2ff11c95be\", \"start_char\": 0, \"end_char\": 1077, \"text_sha256\": \"0bb37e6a5f3624dc13eb87705f21721a6cf7cc2151b0ecf87dedef2ff11c95be\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Intestinal transporter and phosphorylation assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Oral L-theanine supplementation in the mouse experiment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Transporter expression and mTOR pathway activation were associated. Without an intervention blocking the proposed pathway, dependency is not established; no human protein-absorption benefit inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Theanine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mice","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Each transporter remains searchable separately from the overall amino acid response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[theanine-p36615799] L-Theanine Regulates the Abundance of Amino Acid Transporters in Mice Duodenum and Jejunum via the mTOR Signaling Pathway. (2022). https://pubmed.ncbi.nlm.nih.gov/36615799/ DOI: 10.3390/nu15010142","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Intestinal amino acid transport and mTOR signaling","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"59b91d4b-6b22-5bc7-b4c3-748fe99e1dbb","evidence_kind":"source_excerpt","locator":"Lines 640-651","start_line":640,"end_line":651,"excerpt":"### theanine-mouse-intestinal-slc1a3\nThe study reported increased mRNA and protein expression of the mouse intestinal transporter component SLC1A3.\nCondition category: normal\nnutrient_topic: L-Theanine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Each transporter remains searchable separately from the overall amino acid response.\norganism: Mice\ntissue_or_cell_type: Intestinal amino acid transport and mTOR signaling\nexperimental_model: Intestinal transporter and phosphorylation assays\nlimitations: Transporter expression and mTOR pathway activation were associated. Without an intervention blocking the proposed pathway, dependency is not established; no human protein-absorption benefit inferred.\nexposure: Oral L-theanine supplementation in the mouse experiment\nevidence_span: {\"source_cache\": \"artifacts/theanine-research/36615799.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0bb37e6a5f3624dc13eb87705f21721a6cf7cc2151b0ecf87dedef2ff11c95be\", \"start_char\": 0, \"end_char\": 1077, \"text_sha256\": \"0bb37e6a5f3624dc13eb87705f21721a6cf7cc2151b0ecf87dedef2ff11c95be\"}\n[theanine-p36615799] L-Theanine Regulates the Abundance of Amino Acid Transporters in Mice Duodenum and Jejunum via the mTOR Signaling Pathway. 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